introduction: the development of an effective vaccine against hepatitis c virus (hcv) is still a target of intensive vaccine research. the hcv envelope proteins e1 and e2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. different types of expression systems have been used to express these glycoproteins. in this study, an expression system using pichia p...

Background: The Rh antigens are expressed as parts of a protein complex in the RBC membrane. This complex is a tetramer, consisting of two molecules of RhAG and two molecules of Rh proteins. To express RhD in RBC membrane, expression of RhAG is essential. This co-expression only occurs in the erythroid lineage. K562 cell line has an erythroid lineage. Materials and Methods: Cord blood was used ...

Full-length cDNAs encoding macrophage inhibitory factor-2 (MIF-2) were cloned from Teladorsagia circumcincta (TcMIF-2) and Haemonchus contortus (HcMIF-2). TcMIF-2 HcMIF-2 cDNA (342 bp) encoded proteins of 114 amino acids, each which was present as a single band about 16 kDa on SDS-PAGE. Multiple alignments the protein sequences showed homology 84% between HcMIF-2, 54–76% with MIF-2s seven nemat...

Abstract The production of peptides as active pharmaceutical ingredients (APIs) by recombinant technologies is emerging interest. A reliable platform, however, still missing due to inherent peptide characteristics such proteolytic sensitivity, aggregation, and cytotoxicity. We developed a new approach — Numaswitch™. It solves present limitations provides cost-efficient platform for diverse hard...

conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...

Abstract Background The interest in using methanol as a substrate to cultivate acetogens increased recent years since it can be sustainably produced from syngas and has the additional benefit of reducing greenhouse gas emissions. Eubacterium limosum is one few that utilize methanol, genetically accessible and, therefore, promising candidate for recombinant production biocommodities this C1 carb...

a self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pl promoter, was constructed. the designed 5024 base pair expression plasmid contained a heat sensitive repressor ci857 coding gene to regulate the function of ?pl promoter under heat shock induction. using the constructed vector,...

The living cell possesses extraordinary molecular and biochemical mechanisms by which to recognize efficiently remove foreign, damaged, or denatured proteins. This essential function has been a barrier the overexpression of recombinant proteins in most expression systems. A notable exception is E. coli proteins, which, however, end-up as inclusion bodies, i.e., cytoplasmic aggregates that are i...

Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....

Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....