in this study, 42 samples of silage were collected in qaemshahr city in iran during fall 2011. samples were tested by competitive elisa for total aflatoxins. seven samples (16.7%) (mean±se:1.24) from 42 silage samples were positive with total aflatoxin (1.1-27.3 ppb) in nov., dec. and oct. the highest contamination was observed in oct., three samples (21.4%) from 14 samples were contaminated wi...

The purpose of this study was to examine the occurrence of sheep persistently infected with Border disease virus (BDV) on 76 mixed cattle and sheep farms and whether seroconversion to BDV infection occurred in cattle of these farms. Seroprevalence of BDV and bovine viral disease virus (BVDV) infection in sheep was also investigated. Quantitative RT-PCR for pestivirus detection and an ELISA to d...

BACKGROUND Bovine viral diarrhoea (BVD) is considered eradicated from Denmark. Currently, very few (if any) Danish cattle herds could be infected with BVD virus (BVDV). The Danish antibody blocking enzyme-linked immunosorbent assay (ELISA) has been successfully used during the Danish BVD eradication program, initiated in 1994. During the last decade, the cattle herd size has increased while the...

results based on our results, mab against recombinant erns was produced and reacted successfully with recombinant and natural antigens. conclusions with regards to the role of erns in the identification of pi animals, it appears that erns recombinant antigen and the specific monoclonal antibodies produced against it may be suitable for developing bvdv laboratory diagnostic assays. objectives er...

Bovine virus diarrhoea virus (BVDV) has been (tentatively) identified by electron microscopy in purified virus preparations, in infected cell cultures and in tissues and cells from infected animals. These studies have revealed a spherical membrane-bound particle with a diameter of 40-60 nm. The membrane is smooth, bilaminar and surrounds a dense or semi-dense core of 20-25 nm. The core particle...

Two systems of sandwich enzyme-linked immunosorbent assay (ELISA), a two-site monoclonal antibody sandwich ELISA MAb-MAb sandwich ELISA) and a two site polyclonal-monoclonal antibody sandwich ELISA (PAb-MAb sandwich ELISA) for the detection of Plasmodium vivax antigens were developed. The assays showed good correlation with the level of parasitemia when tested against serially diluted P. vivax ...