In atomic force microscopy (AFM) a sharp cantilever tip is used to scan surfaces at the atomic level. One further application is force spectroscopy, in which force-distance curves between binding partners located on the cantilever and substrate surface are determined. This requires specifically immobilized molecules. Herein we describe the covalent binding of single adenosine and thymidine nucl...

Background The aim of the study was to compare the mini-open to the classic open repair techniques for Achilles tendon ruptures. Methods We retrospectively evaluated at a minimum of 2 years follow-up 32 subcutaneous Achilles tendon ruptures; 17 underwent mini-open surgery (Group A) and 15 the open technique (Group B). Results No difference in Achilles Tendon Total Rupture Score and in modif...

BACKGROUND The time required to harvest plant crops is important to the plant properties. It is affected by design of the harvest equipment and the desire for high-quality products with low energy usage. MATERIALS AND METHODS Strength characteristics of Origanum onites L., an important medicinal aromatic plant, harvested on 2, 9, 16 and 23 July, 2012-2014 (H1, H2, H3 and H4, respectively) wer...

Dynamic force spectroscopy (DFS) makes it possible to investigate specific interactions between two molecules such as ligand-receptor pairs at the single-molecule level. In the DFS method based on the Bell-Evans model, the unbinding force applied to a molecular bond is increased at a constant rate, and the force required to rupture the molecular bond is measured. By analyzing the relationship b...

A theory of the unzipping of double-stranded DNA is presented and is compared to recent micromanipulation experiments. It is shown that the interactions that stabilize the double helix and the elastic rigidity of single strands simply determine the sequence-dependent approximately 12-pN force threshold for DNA strand separation. Using a semimicroscopic model of the binding between nucleotide st...

In the permanent magnet linear synchronous motor (PMLSM), force ripple is harmful, useless and disturbing. The force ripple is basically composed of two components: detent force and mutual force ripple. This force is influenced by the geometric parameters of the permanent magnet (PM) motors; such as width, thickness and length of the magnet poles, length and thickness of the rotor and stato...

Type IV pili play an important role in bacterial adhesion, motility, and biofilm formation. Here we present high-resolution atomic force microscopy (AFM) images of type IV pili from Pseudomonas aeruginosa bacteria. An individual pilus ranges in length from 0.5 to 7 microm and has a diameter from 4 to 6 nm, although often, pili bundles in which the individual filaments differed in both length an...

Background: A traumatic diaphragm rupture presents a unique obstacle to a minimally invasive surgical approach; most repairs are performed during an emergency laparotomy. Diaphragm injuries are diagnosed in the acute phase of blunt-force trauma in only 10% of cases, and a high index of suspicion must be maintained to avoid strangulation of the abdominal organs that have herniated into the thora...