A dot-blot, enzyme-linked immunosorbent method and a latex agglutination test were studied for their abilities to detect Vibrio cholerae serotype O1 in aquatic samples by testing artificially contaminated water as well as samples from natural potential sources. Water samples were preenriched with alkaline peptone and then enriched with Monsur peptone water. For the dot-blot test, enriched cultu...

Objective Dot Blot (DB) assay provides highly specific results, but usually not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to be done to develop a quantitative DB assay. Material and Methods Dot blot (DB) strips for antibodies directed to human immuno...

Persistent high-risk (HR) human papillomavirus (HPV) infection is an essential risk factor for cervical carcinoma and precancerous lesion. There are differences in HPV distribution among different countries, regions ethnic groups. The aim of this research was to reveal the epidemiological characteristics Chongqing, China. In study, 13,788 women aged 18 78 were screened 23 genotypes by PCR-rever...

BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. Congenital toxoplasmosis is more important during pregnancy for the first time. In this study we expressed and purified P43 Toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. METHODS The recombinant pGEMEX-1 contained Toxoplasma P43 coding...

Rice stripe virus (RSV) is a plant virus, and its infection leads to devastating disease that often causes serious yield losses in rice-producing regions. In this study, the capsid protein (CP) gene of RSV was cloned expressed E. coli (Rosetta), purified recombinant His-CP used immunize New Zealand white rabbits . After six weeks, polyclonal antibody against (PAb-CP ) obtained from serum immuni...

Virulence of the opportunistic yeast, Candida albieans, involves the interplay of many complex changes including the yeast-hyphae transition, which mainly involves protein changes. Cell wall mannoproteins are found to be the main cause of adherence of C. albieans to epithel ial cells in the first step of an infection process. In the present study, cell wall mannoproteins of intact yeast wer...