From the time the Leishman stain has been used for staining blood cells and parasites, it has been dissolved in acetone-free good quality methyl alcohol. Dissolving the stain in inferiorquality methyl alcohol or ethyl alcohol always gives poor results and hsematologists and parasitologists requiring precision work would always use acetone-free methyl alcohol of the best quality. After the secon...

Latar Belakang: Rhodamin B ditetapkan sebagai salah satu zat pewarna berbahaya oleh Pemerintah Indonesia melalui Peraturan Menteri Kesehatan No.239/MenKes/Per/V/1985 dan sesuai Kepala Badan Pengawas Obat Makanan No.18 tahun 2015 tentang kosmetik, beberapa warna dilarang penggunaannya dalam sediaan kosmetik termasuk lipstik, antara lain B. menyebabkan iritasi serta memberikan efek buruk pada bib...

A fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids resulted in a > 500-fold enhancement in fluoresc...

Aim: A reliable diagnosis of Helicobacter pylori (H. pylori) is important in clinical practice and research. The aim this study was to compare the sensitivity specificity Giemsa stain with hematoxylin eosin (H&E), Toluidine blue, Gimenez Warthin-Starry detection gastric biopsy also immunohistochemistry (IHC) H. organism biopsy. Method: retrospective cross-sectional included 200 formalin-fix...

“Is it possible to predict expression levels of different genes at a given spatial location in the routine histology image tumor section by modeling its stain absorption characteristics?” In this work, we propose “stain-aware” machine learning approach for prediction transcriptomic gene profiles using digital pathology Hematoxylin & Eosin (H&E) section. Unlike recent deep methods which are used...

To optimize diagnosis of histoplasmosis in tissue sections, 30 spleen specimens from mice, experimentally infected with Histoplasma capsulatum, were examined by H&E, Grocott stain, anti-bacille Calmette-Guerin antibody immunostain, Fungiqual A fluorochrome stain (Drs Reinehr and Rembold, Kandern, Germany), and a nested polymerase chain reaction (PCR) assay. Results were compared with the tissue...