نتایج جستجو برای: rk39

تعداد نتایج: 191  

Journal: :The American journal of tropical medicine and hygiene 2012
Subodh Kumar Dinesh Kumar Jaya Chakravarty Madhukar Rai Shyam Sundar

Despite several drawbacks, rK39-based rapid immunochromatographic test is widely used for the diagnosis of visceral leishmaniasis (VL) in the Indian subcontinent. There is an urgent need to develop a better antigen. In this study we separated crude soluble antigens of Leishmania donovani by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hybridized with pool sera from pre- and pos...

Journal: :WHO South-East Asia journal of public health 2012
Syed M Arif Ariful Basher Mohammad R Rahman Mohammad A Faiz

Visceral leishmaniasis (kala-azar) continues to be a major rural public health problem in Bangladesh. A cross-sectional study was carried out in two subdistricts of Mymensingh district from January 2006 to June 2007 to evaluate the delay kala-azar treatment. Suspected patients who attended to out patient department (OPD) were subjected to a dipstick test (RK39) for kala-azar. Sixty five from Bh...

Journal: :Turkiye parazitolojii dergisi 2008
Pinar Yazici Levent Yeniay Unal Aydin Meltem Taşbakan Omer Ozütemiz Rasih Yilmaz

Visceral leishmaniasis (VL) is one of the parasitic infections causing different pathogeneses of various systems including intraabdominal solid organs. L. donovani and L.infantum, particularly in Turkey, have been diagnosed in systemic infections. In the present case study, a 43-year-old woman with left upper abdominal pain, persistent fever and splenic pathology according to the radiological f...

2017
Stefania Varani Margherita Ortalli Luciano Attard Elisa Vanino Paolo Gaibani Caterina Vocale Giada Rossini Roberto Cagarelli Anna Pierro Patrizia Billi Antonio Mastroianni Simona Di Cesare Mauro Codeluppi Erica Franceschini Fraia Melchionda Marina Gramiccia Aldo Scalone Giovanna A Gentilomi Maria P Landini

The diagnosis of visceral leishmaniasis (VL) remains challenging, due to the limited sensitivity of microscopy, the poor performance of serological methods in immunocompromised patients and the lack of standardization of molecular tests. The aim of this study was to implement a combined diagnostic workflow by integrating serological and molecular tests with standardized clinical criteria. Betwe...

2015
Sarah P. Georgiadou Konstantinos P. Makaritsis George N. Dalekos

Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. It is transmitted by phlebotomine female sand flies of the genera Phlebotomus and Lutzomyia in the old and new world, respectively. More than 20 well-recognized Leishmania species are known to infect humans and cause visceral (VL), cutaneous (CL) and mucocutaneous (ML) forms of the disease. Approximat...

Journal: :The Indian journal of medical research 2005
P K Sinha K Pandey S K Bhattacharya

Leishmaniasis, a globally prevalent parasitic disease occurs in three forms viz., visceral, cutaneous and mucocutaneous, transmitted by the bite of infected female Phlebotomus sandflies. Visceral leishmaniasis (VL) has 100 per cent fatality rate, if left untreated. India has the largest burden of this disease. HIV infection is also increasing worldwide and several reports indicate rising trend ...

Journal: :Journal of clinical microbiology 2011
Yousr Galaï Najla Chabchoub Meriem Ben-Abid Imène Ben-Abda Nissaf Ben-Alaya-Bouafif Fethi Amri Karim Aoun Aïda Bouratbine

Current methods for diagnosis of visceral leishmaniasis (VL) require invasive sampling procedures such as visceral aspiration and/or blood drawing. The use of diagnostic tests using oral fluid, which is easier to collect, would be more simple and practical for VL diagnosis, especially under field conditions. Oral fluids from 37 VL patients and 40 healthy controls were collected using Oracol dev...

Abdelhafeiz Mahamoud Abdollah el Harith, Durria Mansur Elfadil Abass Mehdi Mohebali

Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-...

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