This data article contains supporting information regarding the research article entitled "Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm"(Mosca et. al., 2016) [1]. The combined effect of the permeant cryoprotectants agent dimethylacetamide and the non-permeant cryoprotectants agent trehalose on the quality of frozen-thawed chicken sem...

In the United States, poultry genetic resources have consolidated because of economic pressures. Such consolidations can potentially jeopardize the poultry industry and the ability of research communities to respond to future challenges. To address the loss of genetic resources for all livestock and aquatic species, USDA established the National Animal Germplasm Program (NAGP) in 1999. Since th...

Creating transgenic mammals is currently a very inefficient process. In addition to problems with transgene integration and unpredictable expression patterns of the inserted gene, embryo loss occurs at various developmental stages. In the present study, we demonstrate that this loss is due to chromosomal damage. We examined the integrity of chromosomes in embryos produced by microinjection of p...

Two independent experiments were designed to determine the number of sperm needed for maximum fertility of chicken semen processed for freezing. Pooled semen samples were either diluted 1:2 or 1:5 with the Beltsville Poultry Semen Extender, while undiluted samples served as controls. Samples were then subsequently processed (pre-freeze), according to the Beltsville method, as if they were going...

The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species such as pigs whose ooplasm is opaque and pronuclei are often invisible. In this study, the effects of sperm membrane disruption and electrical activation of oocytes on in vitro development and expr...

In ritual practice, which is the part of celebration annual holidays, Serbs usually choose different types animals. Thus, a pig most often sacrificed on Christmas, St. George?s Day - lamb, Elijah rooster, Mrata chicken, etc. The slaughter certain animal, carried out by wider community day year, in Bulgarian-Macedonian-Serbian area known as kurban. paper discusses animal species are particular h...

The chicken reproductive system is unique in maintaining its functions including production of eggs or sperm, fertilization of the egg by sperm maintained in sperm nests, production of hormones regulating its growth, development and function, and reproduction. Development of the reproductive organs is a highly regulated process that results in differentiation and proliferation of germ cells in ...