Strains of Caulobacter crescentus express a paracrystalline surface layer (S-layer) consisting of the protein RsaA. Mutants of C. crescentus NA1000 and CB2, isolated for their ability to grow in the absence of calcium ions, uniformly no longer had the S-layer attached to the cell surface. However, RsaA was still produced, and when colonies grown on calcium-sufficient medium were examined, large...

Many archaeal proteins undergo posttranslational modifications. S-layer proteins and flagellins have been used successfully to study a variety of these modifications, including N-linked glycosylation, signal peptide removal and lipid modification. Use of these well-characterized reporter proteins in the genetically tractable model organisms, Haloferax volcanii, Methanococcus voltae and Methanoc...

We consider the following superlinear elliptic equation on Sn  ε∆Snu− u+ f(u) = 0 in D; u > 0 in D and u = 0 on ∂D, where D is a geodesic ball on Sn with geodesic radius θ1, and ∆Sn is the Laplace-Beltrami operator on Sn. We prove that for any θ ∈ ( 2 , π) and for any positive integer N ≥ 1, there exist at least 2N + 1 solutions to the above problem for ε sufficiently small. Moreover, the asym...

We have established that the gene which we had previously identified as encoding the Methanococcus voltae P-type ATPase is, in fact, the structural gene for the M. voltae S-layer protein. This conclusion is based on a comparison of the N-terminal sequence of S-layer protein prepared by two independent methods with that derived from the nucleotide sequence of the cloned gene. This conclusion was...

We propose a novel high-speed stream cipher encryption scheme based on the true random key generated by a chaotic semiconductor laser. A 5-Gbits/s non-return-to-zero plaintext is successfully encrypted and decrypted using this cryptography. The scheme can be applied in the areas of real-time high-speed physical encryption.

In this paper, we are concerned with the performance at the media access control (MAC) layer of the protocol stack when multibeam adaptive antenna arrays are employed at the base station site. Specifically, we analyze the performance of slotted ALOHA when the base station receiver uses multibeam antenna arrays capable of steering the beams selectively on smaller sectors. The effect of different...

Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments encoding the cell wall-targeting domain of the S-layer protein EA1 and tetanus...

Crystalline cell surface layer proteins (S-layers) can be used in electrochemical fabrication to create nanoscale arrays of metals and oxides on surfaces so long as the proteins maintain their long-range order during processing. We have explored the stability of the HPI layer protein (the S-layer protein from the microorganism Deinococcus radiodurans) adsorbed onto platinum surfaces after immer...

The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridizatio...

Lactobacillus brevis possesses a surface layer protein (SlpA) with tightly regulated synthesis. The slpA gene is expressed by two adjacent promoters, P1 and P2. The level of P2-derived transcripts was approximately 10 times higher than that of P1-derived transcripts throughout the entire growth of L. brevis. The half-lives of slpA transcripts were shown to be exceptionally long (14 min).