نتایج جستجو برای: saccharomyces cerevisiae exoression vector
تعداد نتایج: 238576 فیلتر نتایج به سال:
Background and Aims The use of non-Saccharomyces yeast strains as starter cultures for wine production has become increasingly popular, particularly due to their positive effect on composition, colour, aroma flavour. Here, we characterise the volatile composition sensory profile Shiraz Cabernet Sauvignon wines produced with novel active dry preparations Metschnikowia pulcherrima compared that r...
The mitochondrial tricarboxylic acid cycle enzyme malate dehydrogenase was purified from Saccharomyces cerevisiae, and an antibody to the purified enzyme was obtained in rabbits. Immunoscreening of a yeast genomic DNA library cloned into a lambda gt11 expression vector with anti-malate dehydrogenase immunoglobulin G resulted in identification of a lambda recombinant encoding an immunoreactive b...
Saccharomyces cerevisiae and its sibling species Saccharomyces paradoxus are known to inhabit temperate arboreal habitats across the globe. Despite their sympatric distribution in the wild, S. cerevisiae is predominantly associated with human fermentations. The apparent ecological differentiation of these species is particularly striking in Europe where S. paradoxus is abundant in forests and S...
Yeast researchers need model systems for ecology and evolution, but the model yeast Saccharomyces cerevisiae is not ideal because its evolution has been affected by domestication. Instead, ecologists and evolutionary biologists are focusing on close relatives of S. cerevisiae, the seven species in the genus Saccharomyces. The best-studied Saccharomyces yeast, after S. cerevisiae, is S. paradoxu...
Saccharomyces cerevisiae plays a main role in the winemaking process, although other species, like Saccharomyces uvarum or Saccharomyces paradoxus, have been associated with must fermentations. It has been reported in recent years, that yeast hybrids of different Saccharomyces species might be responsible for wine productions. Although S. cerevisiae×Saccharomyces kudriavzevii hybrids have been ...
We tested 59 Greek patients with Behcet's Disease (BD) for serum anti-Saccharomyces cerevisiae antibodies. No increase of these antibodies was detected in the cases compared to 55 healthy unrelated blood donors from the same population. This finding is in contrast with the correlation between Saccharomyces cerevisiae antibodies and BD as reported in other populations. It seems that environmenta...
Saccharomyces cerevisiae is a commensal inhabiting the gastrointestinal tract of humans, considered important to maintain the normal homeostasis of the lower gastrointestinal tract. A vast majority of patients with AIDS and low CD4 counts present with manifestations involving the gastrointestinal tract in the form of oral thrush, oesophagitis, duodenitis, jejunitis, colitis, and protracted diar...
Yeast prions are infectious proteins that spread exclusively by mating. The frequency of prions in the wild therefore largely reflects the rate of spread by mating counterbalanced by prion growth slowing effects in the host. We recently showed that the frequency of outcross mating is about 1% of mitotic doublings with 23-46% of total matings being outcrosses. These findings imply that even the ...

 Banana peels belong to lignocellulosic substrates, which can be used as raw material for bioethanol. Yeast plays an essential role in bioethanol fermentation. The study aims compare ethanol yield using two different yeasts: Saccharomyces cerevisiae and Rhizopus oryzae, with yeast concentrations of 2, 3, 5 g/L. fermentation is run at room temperature 120 hours. Results show that R. oryzae...
A novel expression vector constructed from genes of Pichia pastoris was applied for heterologous gene expression in Saccharomyces cerevisiae. Recombinant streptokinase (SK) was synthesized by cloning the region encoding mature SK under the control of glyceraldehyde 3-phosphate dehydrogenase (GAP) promoter of Pichia pastoris in Saccharomyces cerevisiae. SK was intracellularly expressed constitut...
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