Gene expression is the process by which inheritable information from a gene, such as the DNA sequence, is made into a functional gene product, such as protein or RNA. Several steps in the gene expression process may be modulated, including the transcription step and translation step and the post-translational modification of a protein. A wide range of microorganisms produce invertase and can, t...

Saccharomyces cerevisiae (S. cerevisiae) (commonly known as baker's yeast) is a model organism that has a similar upstream base excision repair (BER) pathway for the repair of methylated bases as that in mammalian cells, and it is very easy to maintain in the laboratory environment. Here, we described a method to prepare cell extracts from yeast to investigate their enzymatic activities. This p...

Studies about hypoxia-induced oxidative stress in human health disorders take advantage from the use of unicellular eukaryote models. A widely extended model is the fermentative yeast Saccharomyces cerevisiae. In this paper, we describe an overview of the molecular mechanisms induced by a decrease in oxygen availability and their interrelationship with the oxidative stress response in yeast. We...

Non-Saccharomyces yeasts have been used for many years due to their technological potential, particularly as a “booster” of wine fruity aroma in mixed fermentations with Saccharomyces cerevisiae. Recently, new application has emerged, bioprotection, which consists colonizing the environment context sulfite reduction wines. The chemical and sensory impact non-Saccharomyces yeast according differ...

This thesis presents a computational and algorithmic method for the analysis of high-resolution transcription data in the budding yeast Saccharomyces cerevisiae. We begin by describing a computational system for storing and retrieving spatiallymapped genomic data. This system forms the infrastructure for a novel algorithmic approach to detect and recover instances of same-strand overlapping tra...

This work studies the variability of Saccharomyces cerevisiae present during spontaneous fermentation Garnacha grapes’ musts from a “Pago” winery east Spain. The parameters used to select yeast are those related growth, fermentative behaviour, and influence on wine’s aroma polyphenolic composition. Yeast identification was performed by ITS analysis typed Hinfl mDNA restriction profile analysis....

Saccharomyces cerevisiae I-328 is a yeast strain used for production of sherry-like wine in Russia. Here we report the draft genome sequence of this strain, which will facilitate comparative genomic studies of yeast strains used for winemaking.

In the present investigation Saccharomyces cerevisiae was isolated from Toddy and identified by based on morphological and sugar fermentation tests. In alcohol tolerance test, Saccharomyces cerevisiae tolerate up to 15% of ethanol in the medium. Optimize the culture conditions of yeast strain such as initial sugar concentration, pH, temperature and incubation period on ethanol production was al...

the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...

In this study, twenty-five whey samples collected from dairy industries in the city of Isfahan. The sampleswere cultured on malt extract broth (MEB) and yeast extract glucose chloramphenicol agar (YGCA) media.Eleven yeast strains (designated M1 to M11) were isolated from the culture. The strains were identified bytheir morphological and physiological properties. Betagalactosid...