this study was carried out to investigate the morphological and anatomical aspects of somatic embryogenesis in date palm. lateral bud and shoot tip explants excised from young offshoots were cultured on ms medium with 2,4-d. somatic embryogenesis was in-duced by transferring the calli produced on the same medium without hormones. micro-tome sectioning of paraffin-embedded specimens was carried ...

Abstract Scots pine exhibits variations in ray anatomy, which are poorly understood. Some parenchyma cells develop thick and lignified cell walls before heartwood formation. We hypothesized that some stands trees show high numbers of thick-walled early the sapwood. Therefore, a microscopic analysis sapwood from four different Northern Europe was performed on Safranin — Astra blue-stained tangen...

Abstract Transient receptor potential vanilloid 4 (TRPV4) plays an important role in chondrocytes via Ca 2+ signaling. However, its the progression of osteoarthritis is unclear. This study aimed to evaluate effects TRPV4 activation on articular cartilage and stimulated with interleukin (IL)-1?. Bovine human were various agents, including IL-1?, GSK1016790A (GSK101; a agonist), Compound C (an AM...

A comparative study of leaf epidermal features selected species the family Sapindaceae in Nigeria was undertaken with a view to obtain additional characters for elucidation taxonomic relationships, delimiting and identification taxa. The epidermises were separated from mesophyll by soaking concentrated Nitric acid. Bubble appearance on surfaces leaves indicated their readiness separation. They ...

Articular cartilage degeneration represents one of the main features osteoarthritis. Recently, novel approaches based on biomaterials have been successfully applied to osteochondral regeneration. Our study was carried out rabbits assess a model articular damage test for We created defects surface trochlear groove area femurs in 15 white male New Zealand size 3 mm × (diameter depth). Rabbits wer...

As the connecting tissue between the hyaline articular cartilage and the subchondral bone, calcified cartilage zone (CCZ) plays a great role in the force transmission and materials diffusion. However, the questions that remain to be resolved are its mineral composition and organization. In this study, 40 healthy human knee specimens were harvested; first the CCZ was dissected and observed by Sa...

Effects of subcultivation of human bone marrow mesenchymal stem cells on their capacities for chondrogenesis and supporting hematopoiesis, and telomea length were investigated. Mesenchymal stem cells were isolated from human bone marrow aspirates and subcultivated several times at 37 degrees C under a 5% CO(2) atmosphere employing DMEM medium containing 10% FCS up to the 20th population doublin...

OBJECTIVE Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro. DESIGN Scaffolds were genera...

The aim of this study was to observe the effects of a sliding plate on the morphology of the epiphyseal plate in goat distal femur. Eighteen premature female goats were divided randomly into sliding plate, regular plate and control groups. Radiographic analysis and histological staining were performed to evaluate the development of epiphyseal plate at 4 and 8 weeks after surgery. In the sliding...