Abstract Nanocomposites based on acrylonitrile butadiene rubber (NBR) and ethylene–proplene diene monomer (EPDM) (50/50) filled with different concentrations of nano-waste magnetic filler (nWMF) up to 120 phr were prepared by two laboratory roll mill mixer. nWMF was characterized using XRD, TEM VSM. The nanocomposites investigated techniques: SEM, TGA, DSC. In addition the rheological, mechanic...

Abstract This study aimed to investigate the effect of epoxidation levels on curing and tensile properties epoxidized natural rubber (ENR)/microfibrillated cellulose (MFC) composites. ENRs with different were first prepared from (NR) latex via ‘in situ ’ performic using 0.75 M formic acid 1 hydrogen peroxide at 50°C various reaction times. The results H NMR revealed that 2, 4, 6 hours could ach...

We have developed a multilocus sequence typing (MLST) system for identifying the known pathovars of Xylella fastidiosa (including subsp. fastidiosa that causes Pierce’s disease). This identification system is based on allelic variation at seven housekeeping genes (holC, nuoL, leuA, gltT, cysG, petC and lacF) and a public MLST database has been established at www.mlst.net (see Scally et al. 2005...

Epidemiological studies of Pierce’s disease (PD) can be confounded by a lack of genetic information on the bacterial causative agent, Xylella fastidiosa (Xf). PD in grape is caused by genetically distinct strains of Xf subsp. fastidiosa (Xff), but is not caused by numerous other strains or subspecies of Xf that typically colonize plants other than grape. Detection assays such as ELISA and qPCR ...

ABSTRACT Almond leaf scorch disease (ALSD) has recently reemerged in the San Joaquin Valley of California threatening almond production. ALSD is caused by Xylella fastidiosa, a nutritionally fastidious bacterium. Single nucleotide polymorphisms (SNPs) in the 16S rRNA gene (16S rDNA) of X. fastidiosa strains were identified to characterize the bacterial population in infected trees. Genotype-spe...

A full-length cDNA of the genome of Beet black scorch virus (BBSV), isolate Ningxia, was constructed and modified by site-directed mutagenesis to permit in vitro transcription of mutant viral RNAs. Two subgenomic (sg) RNAs (sgRNA1 and sgRNA2) appeared during BBSV replication. Mutagenesis revealed that sgRNA1 transcription was initiated at G2209 within the P82 polymerase subunit open reading fra...

Xylella fastidiosa is the causal agent of almond leaf scorch disease (ALSD), which is currently reemerging in California as a potential threat to almond (Prunus dulcis) production. We previously reported the presence of different colony morphotypes of X. fastidiosa ALSD strains on periwinkle wilt medium solidified with Gelrite and their association with genotypes or pathotypes after a low numbe...