BACKGROUND The swim-up and hyaluronan (HA)-binding methods are used for the selection of good quality spermatozoa to improve pregnancy rates and embryo quality and to reduce the number of miscarriages after IVF. We evaluated whether the processing of sperm by these methods reduces the frequency of spermatozoa with abnormal karyotypes and altered chromatin quality in balanced translocation carri...

Abstract Study question Is the sperm chromatin dispersion (SCD) test reliable for predicting reproductive technology (ART) treatment outcomes? Summary answer Assessment of DNA fragmentation using SCD failed to predict pregnancy or live births rates. What is known already The detrimental effect high on semen quality, fertility, and poor ART outcomes has been well-studied in recent years. Still, ...

Abstract Study question In Individuals with raised Sperm DNA Fragmentation Index (DFI), will sperm selection by magnetic activated cell sorting (MACS) or Microfluidic Sorting (MF) optimize the reproductive outcomes? Summary answer MACS MF offer comparable outcomes. There seems no superiority between them. spermDFI, MACS/MF can be considered to results. What is known already It evident that DFI ...

BACKGROUND Cerium oxide nanoparticles (CeO2 NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO2 NPs for 96 h on the main structural and kinematic parameters of spermatozoa...

BACKGROUND Artificial insemination is not as widely used in horses as in other domestic species, such as dairy cattle and pigs, partly because of the wide variation in sperm quality between stallion ejaculates and partly due to decreased fertility following the use of cooled transported spermatozoa. Furthermore, predictive tests for sperm fertilising ability are lacking. The objective of the pr...

BACKGROUND This case-control study was designed with the aim of evaluating the role of sperm, oxidative stress and DNA damage in idiopathic recurrent pregnancy loss (iRPL). This pilot study is the first study done on the Indian population which reports the association between DFI, TAC and ROS in couples experiencing iRSA. METHODS Twenty infertile men with a history of iRPL and 20 fertile cont...

background: this case-control study was designed with the aim of evaluating the role of sperm, oxidative stress and dna damage in idiopathic recurrent pregnancy loss (irpl). this pilot study is the first study done on the indian population which reports the association between dfi, tac and ros in couples experiencing irsa. methods: twenty infertile men with a history of irpl and 20 fertile cont...

Background Recurrent pregnancy loss (RPL) is defined as the loss of at least three pregnancies in the first trimester. Although the most common cause is embryo aneuploidy, and despite female checkup and couple karyotyping, in about 50% of cases RPL remain unexplained. Male implication has little been investigated and results are discordant. In this context, we conducted a multi-center prospecti...

Diagnosis of sperm DNA integrity of semen sample is important for consistently high reproductive efficiency. The conventional parameters of semen analysis take into account morphology, motility, and concentration of spermatozoa in the sample, which are insufficient for evaluation of reproductive potential. Current studies have implicated abnormal organization of genomic material in sperms as a ...

STUDY QUESTION Do the luminal fluids of the epididymis and the vas deferens contribute to sperm chromatin fragmentation (SCF) in mice? SUMMARY ANSWER The luminal fluids of both organs are required for activating SCF in mice, but the vas deferens luminal fluid does this more efficiently than that of the epididymis. WHAT IS KNOWN ALREADY Mice sperm have the ability to degrade their DNA in an ...