Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods. Methods: S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children’s hospitals. Phenotype analysis for screening v...

The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant production. Continuous culture the most common method grow cells for In study, K12 strain of E. was as production protein. LeExp1 gene plant. Since related found very small amounts plants, it has been reproduced PCR and made workable with this method. T17 vector (...

the aim of this study was to produce a recombinant protein consisting of the catalytic and translocation domains of diphtheria toxin for its later application as a vaccine candidate against corynebacterium diphtheria. to achieve this goal, at first, the amino acid sequence of dt386 was used for prediction of t and b cell epitopes using on-line servers. the dt386 coding sequence was synthesized ...

Our objectives were to determine if milk casein as a percentage of true protein (CN%TP) estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is equivalent CN%TP Kjeldahl, and the proportion (CN), proteolysis products (CNPP), serum (SP) from (TP) that goes into Kjeldahl noncasein nitrogen (NCN) filtrate stays in NCN precipitate using SDS-PAGE. Raw samples collected 1...