Exosomes secreted from Mycobacterium avium-infected macrophages contain numerous antigens of both M. avium and the host cell and are involved in the induction and expression of the inflammatory responses in macrophages. The interaction between exosomes secreted from M. avium-infected macrophages and macrophage phagocytosis, cytokine secretion, immunostimulation, and apoptosis was analyzed. Upon...

Objective: Chemokines are factors secreted from damaged or infected tissues to induce an inflammatory and immunological reaction. Approximately have recently been found be by most cancer cells. The aim of this study is compare the CCR-3 expression with clinicopathological variables in patients prostatic adenocarcinoma(PCa) benign hyperplasia(BPH). Material Method: included 25 PCa who underwent ...

Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 1...

The effects of increased synthesis of secreted proteins expressed from stably integrated heterologous genes in Chinese hamster ovary (CHO) cells following treatment with sodium butyrate was studied. Butyrate treatment increased expression of mRNA transcribed from the adenovirus major late promoter in combination with the SV40 enhancer for Factor VIII, von Willebrand factor, and erythropoietin. ...

Abstract Background Exosomes are membrane-bound extracellular vesicles of 40–150 nm in size, that produced by many cell types, and play an important role the maintenance cellular homeostasis. Exosome secretion allows for selective removal harmful substances from cells. However, it remains unclear whether this process also takes place glioma Methods Herein, tumour-suppressor miR-375 was explored...

Background:  Human coagulation factor IX (hFIX) is a glycoprotein with two N-glycosylation sites at the activation peptide. Since the activation peptide is removed in mature hFIX, the exact role of N-glycosylation is unclear. To investigate the role of N-glycosylation in the secretion and activity of hFIX, we inhibited N-glycosylation by tunicamycin in the stable Human Embryonic Kidney (HEK)- c...

Ashbya gossypii has been recently considered as a host for the expression of recombinant proteins. The production levels achieved thus far were similar to those obtained with Saccharomyces cerevisiae for the same proteins. Here, the b-galactosidase from Aspergillus niger was successfully expressed and secreted by A. gossypii from 2-mm plasmids carrying the native signal sequence at higher level...