A mutant human lysozyme C77/95A, in which Cys77 and Cys95 are replaced with alanine, has been characterized by 8-fold greater secretion in yeast (Taniyama, Y., Yamamoto, Y., Nakao, M., Kikuchi, M., and Ikehara, M. (1988) Biochem. Biophys. Res. Commun. 152, 962-967) and almost the same three-dimensional structure as wild-type human lysozyme (Inaka, K., Taniyama, Y., Kikuchi, M., Morikawa, K., an...

the chemical nature of secretion by the stigma and transmitting tissue in datura stramoniumwas studied by light microscopy using sudan black b, coomasie brilliant blue (cbb), toluidine blue,safranin, and pas. the stigmatic secretion reacted positively for lipids. during development, stigmaticcells became separated and intracellular lipids were transferred to intercellular spaces where theygradu...

background many studies have shown that h2s protects the gastric mucosa on different models of stress but till now there are few studies about the effects of h2s on gastric acid secretion. therefore, the aim of the present study was to investigate the role of autonomic nervous system in mediating the inhibitory effect of hydrogen sulfide donor (nahs) on stimulated gastric acid secretion in rats...

Leptin, a key regulator of fat homeostasis, is the product of the obese gene [1-3], and is secreted from adipocytes and binds to receptor sites in the choroid plexus [4-5]. Several studies have implicated serum insulin levels in the upregulation of leptin gene expression [6-8]. It is currently not known whether leptin levels are also subject to regulation at the level of secretion. Leptin is no...

Camptothecin (CPT) has anticancer, antiviral, and antifungal properties. However, there is a dearth of information about antibacterial activity of CPT. Therefore, in this study, we investigated the inhibitory effect of CPT on Acidovorax avenae subsp. avenae strain RS-2, the pathogen of rice bacterial brown stripe, by measuring cell growth, DNA damage, cell membrane integrity, the expression of ...

Vertebrates differ in their regulation of gastric acid secretion during periods of fasting, yet it is unknown why these differences occur. Elasmobranch fishes are the earliest known vertebrates to develop an acid secreting stomach and as such may make a good comparative model for determining the causative factors behind these differences. We measured gastric pH and temperature continuously duri...

The absorption and splanchnic metabolism of different flavonoids (namely quercetin, kaempferol, luteolin, eriodictyol, genistein, and catechin) were investigated in rats after an in situ perfusion of jejunum plus ileum (14 nmol/min). Net transfer across the brush border ranged widely according to the perfused compound (from 78% for kaempferol to 35% for catechin). This variation seems linked to...

Chinese hamster ovary (CHO) cells are currently the workhorse of the biopharmaceutical industry. However, yeasts such as Pichia pastoris are about to enter this field. To compare their capability for recombinant protein secretion, P. pastoris strains and CHO cell lines producing human serum albumin (HSA) and the 3D6 single chain Fv-Fc anti-HIV-1 antibody (3D6scFv-Fc) were cultivated in comparab...