Enterotoxin E produced by Stu~hyZococcus aureus strain FRI (Food Research Institute)-326 was purified by cation exchange chromatography on carboxymethylcellulose, gel tiltration through superfine Sephadex G-75, and gel filtration in 6 M urea with superfine Sephadex G-75. The purified toxin appears to be nearly homogeneous by paper, starch gel, and polyacrylamide gel electrophoresis and double g...

An anti-epilepsy peptide (AEP) was isolated and purified from venom of the scorpion Buthus martensii Karsch. The purification procedure included CM-Sephadex C-50 chromatography, gel filtration on Sephadex G-50 and DEAE-Sephadex A-50 chromatography. Its homogeneity was demonstrated by pH 4.3 polyacrylamide-disc-gel electrophoresis, focusing electrophoresis and SDS/polyacrylamide-disc-gel electro...

Thyroxine-binding alpha globulin (TBG) in human serum was isolated from Cohn fractions IV-5,6 and IV-4 by (1) chromatography on carboxymethyl (CM) cellulose, (2) gel filtration on Sephadex G-200, (3) chromatography on diethylaminoethyl-Sephadex, (4) a novel procedure of "double-gel" electrophoresis, and (5) preparative polyacrylamide gel electrophoresis. The protein was homogeneous by analytica...

It is known that some strains of Vibrio parahaemolyticus are responsible for gastroenteric diseases caused by the ingestion of marine organisms contaminated with these bacterial strains. Organic products that show inhibitory activity on the growth of the pathogenic V. parahaemolyticus were extracted from a Vibrio native in the north of Chile. The inhibitory organic products were isolated by rev...

Radioactive nickel chloride (63NiC12) was injected i.v. into rabbits in dosage of 0.24 mg Ni/kg body wt. 63Nj was rapidly cleared from the serum (T112 8.2 h) during the period from 1 h to 2 days after the injection, but slowly (T112 95 h) during the period from 4 to 7 days after the injection. During 24 h after injection of 63NiC12, an average of 90% of serum 63Ni was bound to albumin and 10% w...

In the present study, a qualitative analysis of proanthocyanidins (PAs) from an aqueous-methanolic extract of Salix daphnoides VILL. bark is described. Procyanidin B1 (1), B2 (2), B3 (3), B4 (4), C1 (5), epicatechin-(4β→8)-epicatechin-(4β→8)-catechin (6) and epicatechin-(4β→8)-epicatechin-(4β→8)-epicatechin-(4β→8)-catechin (7) have been isolated by a combination of different chromatographic sep...

arthrobotrysamerospora (atcc 34468) produced glucoamylase in a semi-synthetic medium containing starch as a sole carbon source. polyacrylamide gel electrophoresis of crude glucoamylase showed three isoenzymes. they were designated as glu i, glu ii and glu iii according to their electrophoretic mobility. these iso-glucoamylases were purified by column chromatography using deae-sephadex a-50. the...

As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI ...

1. Glycopeptides were isolated by gel filtration on Sephadex G-25 and Sephadex G-50 from a Pronase digest of porcine thyroglobulin. 2. Isolated glycopeptides were separated into five main fractions on a column of DEAE-Sephadex A-25. Of these fractions I to III were further purified by SE-Sephadex C-25 or DEAE-Sephadex A-25 column chromatography. Several of the purified glycopeptides were homoge...

In the preceding paper we reported the synthesis of affinity matrices containing 3,5,3'-triiodo-~-thyonine (T3) Linked via its amino group and the diactivated ester of glutaric acid to the free amino groups of diaminohexane-Sepharose. This report describes the optimization of this system for the purification of the intranuclear thyroid hormone receptor. The thyroid hormone receptors (Kd for T3 ...