1. alpha-Cyano-4-hydroxycinnamate was coupled to Sepharose CL-4B activated with 1,2:3,4-bisepoxybutane. 2. The low-Km rat liver mitochondrial aldehyde dehydrogenase was specifically bound to this affinity medium, and could subsequently be eluted with alpha-cyano-4-hydroxycinnamate. 3. The enzyme purified in this manner had a subunit molecular mass of 55 kDa and a pI of approx. 6.5. A minor comp...

In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of Lentinus tigrinus exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS--C) were purified by Sepharose CL-6B ge...

Monoclonal antibodies against the porcine 1,25-dihydroxyvitamin D3 receptor were immobilized on Sepharose CL-4B and used to obtain a highly purified 1,25-dihydroxyvitamin D3 receptor fraction with a 45% recovery of the 1,25-dihydroxyvitamin D3 binding capacity. The porcine receptor was purified to homogeneity by preparative electrophoresis and digested in sodium dodecyl sulfate/polyacrylamide g...

An endo-beta-glucuronidase acting on chondroitin sulfate was isolated from rabbit liver and purified about 550-fold, using a combination of ammonium sulfate fractionation, DEAE-cellulose chromatography, gel filtration on Sephacryl S-300, affinity chromatography through heparin-Sepharose CL-6B and preparative polyacrylamide gel electrophoresis. The pH optimum of this enzyme was 4.0 and the Km va...

A se}ectiye inactivating enzyme for the m-subunit of lactate dehydrogenase (LDH) was found in the cutture filtrate of PenieiM"m citrinum KE-1, newly isolated from soil. The enzyme was purified from the culture filtrate by ammonium sulfate fractionation, column chromatography on CM-Sepharose CL-6B, and gel filtration on Sephadex G-1OO. The purification was 124-fold with an activity yield of 81%....

A Ca2+-activated cysteine proteinase (calpain II) was purified from chicken gizzard smooth muscle by use of isoelectric precipitation, (NH4)2SO4 fractionation, chromatography on DEAE-Sepharose CL-6B, Reactive-Red 120-agarose and Mono Q. The apparent second-order rate constants for the inactivation of calpain by a series of structural analogues of L-3-carboxy-trans-2, 3-epoxypropionyl-leucylamid...

1. The charge state of two derivatives of Sepharose prepared by the CNBr activation method were studied by acid-base titration and by ion-exchange chromatography. Dodecyl-Sepharose exhibited cationic groups (21 pmol/ml of settled gel; pKa = 9.6) that were tentatively assigned to the coupling isourea group. 2. CPAD-Sepharose (N-(3carboxypropionyl)aminodecyl-Sepharose] has anionic (carboxyl) grou...

Purified motoneurons from chick, cultured on polycationic substrata treated with myotube-conditioned (MCM), respond by rapidly extending neurites. When MCM, partially purified by salt precipitation and ion-exchange chromatography, was fractionated on Sepharose CL-4B, the peak of neurite outgrowth-promoting activity (NOPA) corresponded to a peak of laminin (LA) immunoreactivity. Fractions from t...

molecular size and containing more carbohydrate (80% (w!w)), Many extensive works have been reported on the characterization of the epitopes recognized by HMFG-1 and HMFG2.3,4・7-iO) It has been shown that HMGP-C was recognized by both antibodies9). However, whether each HMGP in hurnan milk carries the epitopes or they occur only in some species has not been elucidated. In addition, the nature o...