Lipidcs extracted from human carcinomas of the stomach, small intestine, and colon wcrc fract ionated on silicic acid columns and the fractions tested for serological act ivi ty with two antisera: one containing anticytolipin II an(] the other devoid of this ant ibody but reactive with crude lipidcs of gastrointestinal tissues, both normal and neoplastic. Two different haptens could be demonstr...

The isolation and identification of the phosphonolipids from beef brain--right lobe, left lobe and cerebellum--is herein reported. The phosphonolipids were isolated by preparative thin layer chromatography (TLC) using the solvent system methanol/water (2:1, v/v) and identified preliminarily by TLC. The isolated phosphonolipids were subjected to silicic acid column chromatographic separation and...

An improved method for the isolation of the biosurfactant glycolipids from Rhodococcus sp. strain H13A by using XM 50 diafiltration and isopropanol precipitation was devised. This procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. The protein apparently does not contribute any biosurfactant char...

Virantmycin, a novel chlorine-containing antiviral antibiotic, has been isolated from Streptomyces nitrosporeus No. AM-2722. The active substance in culture broth is isolated as colorless needles by solvent extraction followed by high performance liquid chromatography on silicic acid. The molecular formula is C19H26NO3Cl (molecular weight 351) from the elemental analysis and mass spectrum. The ...

Urine from patients with ketoacidosis was found to contain a number of aliphatic 3-hydroxy dicarboxylic acids. The acids were purified by silicic acid chromatography and their structures determined by gas chromatography-mass spectrometry of different derivatives. The major compound was 3-hydroxydecanedioic acid. Minor compounds were 3-hydroxyoctanedioic acid, 3-hydroxyoctenedioic acid, 3-hydrox...

1. The total lipid was extracted from Staphylococcus lactis I 3 with chloroformmethanol mixtures and the glycolipid component was isolated by chromatography on silicic acid. 2. Saponification yielded a non-crystalline glycoside for which the structure O-D-glucopyranosyl-(1 -÷6)-O-fi-D-glucopyranosyl-(1 -*1)-D-glycerol has been established by chemical degradations and by comparison with syntheti...

A rapid comprehensive thin-layer chromatographic separation of the major plasma lipid classes on commercial silicic acid glass fiber paper is described. For screening purposes, oxidized fractions are rapidly visualized for qualitative interpretation. Standard colorimetric procedures for each lipid class are per­ formed upon the migration areas cut from the strips. This provides quantitative mea...