The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs is added to the PCR reaction and the PCR products are resolved on PAGE and auto...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission has been reported worldwide and novel SARS-CoV-2 variants continue to emerge. A strain, the Delta variant (B.1.617.2), is spreading worldwide. The reportedly high infectivity immune evasion potency. In June 2021, World Health Organization categorized it as a of concern (VOC). Therefore, vital develop tests that can exclus...

Diagnostic immunoglobulin (Ig) polymerase chain reaction (PCR) clonality analyses need to be simple, reproducible, and rapid. Sucrose and cresol red (gel loading buffer reagents) were added to a routine IgH PCR reaction mix to obviate the need for adding gel loading buffer separately after PCR amplification. Not only did this decrease the time spent after PCR analysis but also gave similar or e...

Myclobutanil is a chemical pesticide commonly used in the production of some vegetables and fruits like greenhouse peppers, grapes, apples. The aim this study was to investigate genotoxic cytotoxic effects myclobutanil fungicide on Allium cepa plant, model organism. Randomly amplified polymorphic DNA inter simple sequence repeat-PCR techniques were performed A. exposed different doses time peri...

Background While whole genome sequencing (WGS) may be more expensive than traditional testing and polymerase chain reaction (PCR), simple cost comparisons ignore the potential for WGS to reduce societal costs of non-typhoidal Salmonella enterica through public health action prevent illness. Methods We determined how many cases use data would need cost-equal serotyping MLVA, or culture independe...

in this paper, we introduce the concept of semihyperring $(r,+,cdot)$ together with a suitable partial order $le$. moreover, we introduce and study hyperideals in ordered semihyperrings. simple ordered semihyperrings are defined and its characterizations are obtained. finally, we study some properties of quasi-simple and $b$-simple ordered semihyperrings.