Size exclusion chromatography (SEC, also called GPC or GFC) is the most important test method for the determination of the molecular properties of synthetic and natural macromolecules in solution. Different from traditional methods which only establish average property values, SEC allows the simple and fast determination of property distributions of macromolecules, e.g. molecular weight distrib...

Full-length huntingtin (FL HTT) is a large (aa 1-3,144), ubiquitously expressed, polyglutamine (polyQ)-containing protein with mass of approximately 350 kDa. While the cellular function FL HTT not entirely understood, mutant expansion polyQ tract above ~36 repeats associated Huntington's disease (HD), length correlating roughly age onset. To better understand effect structure on (mHTT), quantit...

The chromatographic methods used for the purification of PEGylated proteins are mainly Size Exclusion (SEC) and Ion Exchange Chromatography (IEX). Although the PEGylation affects the protein hydrophobicity, Hydrophobic Interaction Chromatography (HIC) has not been extensively applied for the separation of these proteins. Purification of monoPEGylated Ribonuclease A (RNase A) using HIC is studie...

The evaluation of weight average molecular weight (Mw) and molecular weight distribution represents one of the most controversial aspects concerning the characterization of low molecular weight heparins (LMWHs). As the most commonly used method for the measurement of such parameters is high performance size exclusion chromatography (HP-SEC), the soundness of results mainly depends on the approp...

The data provided in this article are related to the research article entitled "Unaided trifluoroacetic acid pretreatment solubilizes polyglutamine (polyGln) peptides and retains their biophysical properties of aggregation" by Burra and Thakur (in press) [1]. This research article reports data from size exclusion chromatography (SEC), reversed phase-high performance liquid chromatography (RP-HP...

The prokaryotic lysine-specific permease (LysP) belongs to the amino acid-polyamine-organocation (APC) transporter superfamily. In the cell, members of this family are responsible for the uptake and recycling of nutrients, for the maintenance of a constant internal ion concentration and for cell volume regulation. The detailed mechanism of substrate selectivity and transport of L-lysine by LysP...

Analytical chemistry of metal complexes with biomacromolecules based on the coupling of a high resolution separation technique with an element or species selective detection technique is critically discussed. The role of size-exclusion chromatography (SEC) with on-line atomic spectrometric detection is evaluated for the characterization of the metal distribution among the fractions of different...

Static light-scattering (LS) detection can determine the molecular weight (MW) of polymers eluted with size-exclusion chromatography (SEC) without using any standards when the differential refraction index (RI) of solutes are obtained. On the other hand, the noisy chromatographic signal peak acquired using a static LS detector often causes difficulty in peak-width recognition. This disadvantage...

High-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with ultraviolet absorbance (UVA) and on-line dissolved organic carbon (DOC) detectors has been adapted and optimized under various conditions. An enhanced HPSEC-UVA system employing a modified commercially available DOC detector provides a better understanding of the qualitative and quantitative natural organic m...

Two scalable polymerisation methods are used in combination for the synthesis of ethylene and methacrylate block copolymers. ω-Unsaturated methacrylic oligomers (MMAn) produced by catalytic chain transfer (co)polymerisation (CCTP) methyl (MMA) acid (MAA) as reagents radical (E) dimethyl carbonate solvent under relatively mild conditions (80 bar, 70 °C). Kinetic measurements analyses copolymers ...