SNPServer is a real-time flexible tool for the discovery of SNPs (single nucleotide polymorphisms) within DNA sequence data. The program uses BLAST, to identify related sequences, and CAP3, to cluster and align these sequences. The alignments are parsed to the SNP discovery software autoSNP, a program that detects SNPs and insertion/deletion polymorphisms (indels). Alternatively, lists of relat...

It is now about 40 years since Avram Goldstein proposed the use of the stereoselectivity of opioid receptors to identify these receptors in neural membranes. In 2012, the crystal structures of the four members of the opioid receptor family were reported, providing a structural basis for understanding of critical features affecting the actions of opiate drugs. This minireview summarizes these re...

A composite genetic linkage map for the yellow fever mosquito Aedes aegypti was constructed based on restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and single strand conformation polymorphism (SSCP) markers. The map consists of 146 marker loci distributed across 205 cM, and includes several morphological mutant marker loci. Most of the genetic markers are ...

BACKGROUND/AIM Recent data have indicated that the rs1080985 single nucleotide polymorphism (SNP) of the cytochrome P450 (CYP) 2D6 and the common apolipoprotein E (APOE) gene may affect the response to donepezil in patients with Alzheimer's disease (AD). We investigated this association in Chinese patients with mild-to-moderate AD. METHODS In this prospective cohort study, analyses of CYP2D6 ...

It is now about 40 years since Avram Goldstein proposed the use of the stereoselectivity of opioid receptors to identify these receptors in neural membranes. In 2012, the crystal structures of the four members of the opioid receptor family were reported, providing a structural basis for understanding of critical features affecting the actions of opiate drugs. This minireview summarizes these re...

OBJECTIVE To investigate the association of the 2 intracellular adhesion molecules-1 (ICAM-1) gene polymorphisms [thymidine/cytidine (T/C) 469 and guanosine/adenosine (G/A) 241] in Behçets disease in Lebanon. METHODS We initiated the study in July 2003, and carried out the work in the research laboratory of Beirut Arab University, Beirut, Lebanon. We extracted the DNA by glass fiber matrix mi...

The mud shrimp Upogebia major (Crustacea, Decapoda, Gebiidea) (De Haan, 1841) is an ecological keystone species widely distributed in waters. It has contributed significantly to the maintenance of coastal benthic biodiversity as ecosystem engineer. However, there still a lack effective molecular markers effectively protect and manage this species. In study, we used DNeasy Blood & Tissue kit ext...

Abstract Genotyping by sequencing (GBS) and single nucleotide polymorphism (SNP) chip technologies are the primary SNP genotyping used today. However, these have some drawbacks that limit their usefulness in analysis. We developed a robust protocol called multiplexed (GMS) using markers, providing informative genotypic data with greater flexibility. The genotypes derived from direct sequence re...

Human choline dehydrogenase (CHDH) is a transmembrane protein located in mitochondria. CHDH has been shown to be one of the important catalytic enzymes that catalyze oxidation betaine and involved mitochondrial autophagy after damage. In recent years, an increasing number studies have focused on found close association with pathogenesis various diseases, including tumor prognosis. Here we summa...

Rice is the staple food in Republic of Guinea. However, its production seriously hampered by iron toxicity. The fight against this stress necessarily involves search for tolerant or resistant varieties. study aims to determine quantitative trait locus (QTL) associated with tolerance toxicity and identify genotypes Thus, 90 local rice accessions collected two regions Guinea high were evaluated u...