The epithelium lining the gallbladder of mammalian species has absorptive and secretory functions. An important function is the secretion of a bicarbonate rich fluid that helps neutralise stomach acid and provides an appropriate environment for intestinal enzymes. In cystic fibrosis (CF) this secretory function is lost. This study concerns the bicarbonate secreting activity of murine gallbladde...

Cytosolic carbonic anhydrase II (CAII) and the cytoplasmic C-terminal tails of chloride/bicarbonate anion exchange (AE) proteins associate to form a bicarbonate transport metabolon, which maximizes the bicarbonate transport rate. To determine whether cell surface-anchored carbonic anhydrase IV (CAIV) interacts with AE proteins to accelerate the bicarbonate transport rate, AE1-mediated bicarbona...

Puziss, Milton (Fort Detrick, Frederick, Md.) and Mary B. Howard. Studies on immunity in anthrax. XI. Control of cellular permeability by bicarbonate ion in relation to protective antigen elaboration. J. Bacteriol. 85:237-243. 1963.-No elaboration of Bacillus anthracis protective antigen was demonstrated after addition of bicarbonate to cultures incubated 42 hr in a bicarbonate-free medium. Ant...

Cytosolic carbonic anhydrase II (CAII) and the cytoplasmic C-terminal tails of chloride/bicarbonate anion exchange (AE) proteins associate to form a bicarbonate transport metabolon, which maximizes the bicarbonate transport rate. To determine whether cell surface-anchored carbonic anhydrase IV (CAIV) interacts with AE proteins to accelerate the bicarbonate transport rate, AE1-mediated bicarbona...

The elevation of pCO2 which occurs during respiratory acidosis is accompanied by a rise in the concentration of bicarbonate and a fall in the concentration of chloride in serum (1). The increased bicarbonate concentration results from both cellular and renal responses. The cellular response is characterized by the exchange of extracellular hydrogen ions for intracellular cations, thereby contri...

PHYSIOLOGICAL AND CHEMICAL CONSIDERATIONS Definitions: Van Slyke72 has defined acidosis as a condition in which the bicarbonate of the blood or serum, or, more exactly, the base available for combination with bicarbonate, is diminished. Alkalosis is a condition in which there is in the blood or serum an abnormal excess of bicarbonate, or base available for combination with bicarbonate. These de...

Our previous studies in cortical collecting ducts isolated from rat kidneys have shown that vasopressin increases both sodium absorption and potassium secretion, while bradykinin inhibits sodium absorption without affecting potassium transport. To determine which anions are affected by these agents, we perfused cortical collecting ducts from rats treated with deoxycorticosterone and measured ne...

During perfusion of a plasma-like solution, colonic absorption rate of chloride was much higher than the secretion rate of bicarbonate (34 vs. 3.5 meq/h, respectively). This might suggest that anion exchange (Cl/HCO3) accounts for only a small fraction of total chloride absorption. However, if the colon absorbs as well as secretes bicarbonate, this reasoning would underestimate the magnitude of...

Evans, R. S., Olver, R. E., Appleyard, W. J., and Newman, C. G. H. (1970). Archives of Disease in Childhood, 45,321. Effects of intragastric and intravenous sodium bicarbonate on rate of recovery from post-asphyxial acidosis in the neonate. A trial was carried out on acidotic infants recovering from neonatal asphyxia, on the relative effects of intragastric and intravenous sodium bicarbonate on...

This study examines endogenous acid production in a group of stable chronic hemodialysis patients with no residual renal function who were admitted to the chronic hemodialysis unit of Mount Sinai Hospital. Net acid production was estimated by the change in body bicarbonate content, which occurs in the interdialytic period. The body bicarbonate content at any time was measured by multiplying the...