By comparing differences among the effects of several isolation mediums on soil actinomycetes, we attempted to find a isolation medium that could result in actionomycetes with high diversity. Soil samples were isolated by the dilution plateing method, and genomic DNA was extracted using phenol-chloroform method. After amplification by 16S rDNA PCR, PCR products were sequenced and undertaken phy...

DNA detection and analysis has become a common way to identify organisms in many different types of samples, including soils that contain organisms potentially capable of biodegrading pollutants. Such analytical procedures, known as DNA diagnostic techniques, generally start with the extraction of DNA from organisms grown under culture conditions in the laboratory. The sequence of the DNA provi...

Rhizoctonia solani anastomosis group AG2-2 IIIB is a severe sugar beet and maize pathogen. It causes crown and root rot disease which le ads to y ield losses world-wide. The soil-borne pathogen is d ifficult to detec t and quantify by conventional methods. We developed a real-time PCR (qPCR) assay for the quantification of genomic DNA of Rhizoctonia solani AG2-2 IIIB based on the ITS region of ...

Forensic crime scene sample analysis, by its nature, often deals with samples in which there are low amounts of nucleic acids, on substrates that often lead to inhibition of subsequent enzymatic reactions such as PCR amplification for STR profiling. Common substrates include denim from blue jeans, which yields indigo dye as a PCR inhibitor, and soil, which yields humic substances as inhibitors....

Low height embankments are widely used as road for highways and rural roads in India. Sometimes, the construction of such types on soft foundation soil becomes mandatory. In addition to problematic soil, seismic excitation might also play a significant role stability an embankment. The present study analyses response low embankment with under earthquake loading. Numerical simulations have been ...

Under most engineering conditions, soil disturbance due to pile installation may cause properties vary within the region adjacent in radial direction. This paper derives a rigorous solution investigate kinematic response of end-bearing piles under excitation vertical P-waves considering construction effect. The displacement responses and soil, governed by dynamic equilibrium equation, are theor...

background and objectives: proline dehydrogenase (prodh; 1.5.99.8) plays an important role in specific determination of plasma proline level in biosensor and diagnostic kits. the goal of this research was to isolate and characterize prodh enzyme from iranian soil microorganisms. materials and methods : screening of l-proline degradative enzymes from soil samples was carried out employing enrich...

Plague is characterized by geographical foci from which it re-emerges after decades of silence, a fact currently explained by enzootic and epizootic cycles between plague-susceptible and plague-resistant rodents. To assess the potential role of soil in plague epidemiology, we experimentally investigated whether Yersinia pestis could persist alive and virulent in soil. Sterilized soil inoculated...