An Actinomycete, strain K-128, which was isolated from soil, formed a transfer product when the strain was cultured in a medium containing both stevioside and curdlan. The transfer product in culture broth was separated by DIAION HP-20 column and TOYOPEARL HW-40F column chromatographies. From structural studies, the transfer product was identified as C13-O-beta-6(2)-beta-glucosylsophorosyl-C19-...

The sequences of the largest subunit of bacterial multicomponent phenol hydroxylases (LmPHs) were compared. It was found that LmPHs formed three phylogenetic groups, I, II, and III, corresponding to three previously reported kinetic groups, low-K(s) (the half-saturation constant in Haldane's equation for trichloroethylene [TCE]), moderate-K(s), and high-K(s) groups. Consensus sequences and spec...

A novel platelet aggregation inhibitor, YM-254890, was isolated from the culture broth of strain QS3666. This strain was isolated from a soil sample collected at Okutama, Tokyo, Japan, and was identified as Chromobacterium sp. by morphological and physiological criteria. YM254890 was purified from the culture supernatant by solvent extraction, ODS and silica gel flash chromatography, followed b...

BACKGROUND Symbioses between metazoans and microbes are widespread and vital to many ecosystems. Recent work with several nematode species has suggested that strong associations with microbial symbionts may also be common among members of this phylu. In this work we explore possible symbiosis between bacteria and the free living soil bacteriovorous nematode Acrobeloides maximus. METHODOLOGY W...

Growth in the presence of sucrose was shown to confer to Arabidopsis thaliana (thale cress or mustard weed) seedlings, under conditions of in vitro culture, a high level of tolerance to the herbicide atrazine and to other photosynthesis inhibitors. This tolerance was associated with root-to-shoot transfer and accumulation of atrazine in shoots, which resulted in significant decrease of herbicid...

1 www.ijeas.org  Abstract— Soil contamination from industrial effluents and mining activities is a widespread concern all over the world. Generally soil act as the buffer zone between atmosphere and underground water, thus soil contamination creates a serious threat to soil and to the surrounding biosphere. This research program is aimed to make an in-depth investigation about the remediation ...

Four-week-old seedlings of Grindelia camporum Greene were planted in a mild calcareous alluvial soil collected from Mahibullapur, in Lucknow district (26 300 N latitude–80 300 E longitude) in pot culture. The soil, rated sulphur deficient on the basis of available soil sulphur, was fertilized with calcium sulphate to provide sulphur at the rate of 10, 25, 50, and 100 mg kg 1 soil. Observations ...

Our previous work demonstrated that application of a bio-organic fertilizer (BIO) to a banana mono-culture orchard with serious Fusarium wilt disease effectively decreased the number of soil Fusarium sp. and controlled the soil-borne disease. Because bacteria are an abundant and diverse group of soil organisms that responds to soil health, deep 16 S rRNA pyrosequencing was employed to character...

A culture independent method based on qPCR was developed for the detection and quantification of two fungal inoculants in soil. The aim was to adapt a genotyping approach based on SSR (Simple Sequence Repeat) marker to a discriminating tracing of two different species of bioinoculants in soil, after their in-field release. Two entomopathogenic fungi, Beauveria bassiana and B. brongniartii, were...

The present study was aimed to isolate, identify and screening of enzyme producing bacteria from soil samples. For this purpose the soil samples were collected from agriculture fields at sangam dairy, Guntur district, fish culture ponds, timber depot soil at rural areas of Bhimavaram, West Godavari district and oil mills at Vijayawada, Krishna district, Andhra Pradesh. A total of seventeen stra...