In this study, we used solid phase synthesis to prepare three kinds of peptides and then formulated their peptide-siRNA complexes and peptide-siRNA conjugates. Both the complexation and conjugation systems were nontoxic and allowed the delivery of siRNA into the cytoplasm without the need for any transfection agents and with subsequent inhibition of gene expression.

Peptides are one of the best candidates for drug development due to their high specificity and low toxicity and porphyrins are significant macromolecules in biological systems with important roles. In this works ynthesis of dipeptide (histidine-β-alanine) was done by solid-phase peptide synthesis method (SPPS) and tetrakis(4-sulfonatophenyl)porphyrin (TPPS4) was synthesized by Adler method. The...

A new biological active hexapeptide of C-terminal of nocistatin, contains Glu-Gln-Lys-Gln-Leu-Gln sequence was synthesized according to solid phase peptide synthesis on the surface of 2-chloro tritylchloride resin and using fmoc-protected amino acids in the presence of TBTU (O-(Benzotriazol-1-yl)-N,N,N',N'-tetramethyl uranium tetrafluoroborate) as a coupling reagent. Then, amidation of the C-te...

introduction: over expression of selected peptide receptors in human tumors has been shown to represent clinically relevant targets for cancer diagnosis and therapy. the aim of this work was to investigate neuropeptide y (npy) as a new radiopharmaceutical for diagnosis of breast cancer. methods: a neuropeptide y analogues with y1 receptor preference and agonistic properties was synthesized by s...

in this work, the strontium-doped lanthanum manganite- a ceramic material- used as cathode in solid oxide fuel cells. an impression of grinding time on the structural and thermal properties of sr-doped lamno3 system with la1-xsrxmno3 (x=0.2) stoichiometric ratio was investigated. the nano crystallite lsm powder with cubic structure was prepared by varying the milling time of planetary monomill ...

This review article focuses on concepts that incorporate safety-catch and multiply cleavable linkers in solid-phase synthesis. Discussed are specific applications of such linkers in the synthesis of peptides, peptide mimetics, and “small” organic molecules, as well as their limitations for particular chemistries and reaction conditions. © 1999 John Wiley & Sons, Inc. Biopoly 47: 353–363, 1998

A strategy for the linear synthesis of a sialylated glycolipopeptide cancer vaccine candidate has been developed using a strategically designed sialyl-Tn building block and microwave-assisted solid-phase peptide synthesis. The glycolipopeptide elicited potent humoral and cellular immune responses. T-cells primed by such a vaccine candidate could be restimulated by tumor-associated MUC1.

[chemical reaction: see text]. A solid phase approach is presented for the synthesis of azapeptide inhibitors and activity based probes (ABPs) for cysteine proteases. This synthetic method allows the incorporation of diverse reactive warheads linked to different peptide recognition elements. Application of this method to the synthesis of a series of caspase probes is described.

Synthesis of Fmoc-protected N(δ)-acetyl-N(δ)-(tert-butoxy)-l-ornithine has revealed it to be a metal-chelating amino-acid precursor. This protected amino acid was compatible with the preparation of ferrichrome peptides by standard Fmoc-based solid-phase peptide synthesis. Evaluation of deferriferrichrysin for metal ion chelation revealed that zirconium(IV) and titanium(IV) formed complexes with...