A 65-year-old male presented with a slowly enlarging nodule on his right leg, which had been present for 10 years and which had gradually increased in size over the previous 6 months. Physical examination revealed a red and dark bluish, dome-shaped nodule, 26 mm × 22 mm in size, on the right side of the posterior aspect of his right leg (Fig. 1). Histopathology revealed a well-demarcated, solid...

OBJECTIVE To find out the prevalence of concomitant active pulmonary Tuberculosis (TB) in patients of Chronic Obstructive Pulmonary Disease (COPD) using the gold standard liquid and solid culture media for the detection of acid fast bacillus. METHODS Eighty clinically and radiologically diagnosed cases of COPD of any severity, ≥40 years of age with no previous history of anti-tuberculous ther...

From the time the Leishman stain has been used for staining blood cells and parasites, it has been dissolved in acetone-free good quality methyl alcohol. Dissolving the stain in inferiorquality methyl alcohol or ethyl alcohol always gives poor results and hsematologists and parasitologists requiring precision work would always use acetone-free methyl alcohol of the best quality. After the secon...

A fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids resulted in a > 500-fold enhancement in fluoresc...

Aim: A reliable diagnosis of Helicobacter pylori (H. pylori) is important in clinical practice and research. The aim this study was to compare the sensitivity specificity Giemsa stain with hematoxylin eosin (H&E), Toluidine blue, Gimenez Warthin-Starry detection gastric biopsy also immunohistochemistry (IHC) H. organism biopsy. Method: retrospective cross-sectional included 200 formalin-fix...

“Is it possible to predict expression levels of different genes at a given spatial location in the routine histology image tumor section by modeling its stain absorption characteristics?” In this work, we propose “stain-aware” machine learning approach for prediction transcriptomic gene profiles using digital pathology Hematoxylin & Eosin (H&E) section. Unlike recent deep methods which are used...