Resonance Raman spectra of ferrous and ferric cytochrome c peroxidase and Compound ES and their pH dependences were investigated in resonance with Soret band. The Fe(IV) = O stretching Raman line of Compound ES was assigned to a broad band around 767 cm-1, which was shifted to 727 cm-1 upon 18O substitution. The 18O-isotopic frequency shift was recognized for Compound ES derived in H218O, but n...

Mn-peroxidase (MnP), a biotechnologically important enzyme was purified for the first time from a plant source Musa paradisiaca (banana) stem, which is an agro-waste easily available after harvest of banana fruits. MnP was earlier purified only from the fungal sources. The enzyme was purified from stem juice by ultrafiltration and anion-exchange column chromatography on diethylamino ethylcellul...

The circular dichroic (CD) behavior of ferriand ferrocytochrome c in the presence of methanol, ethanol, and 1-propanol u-as investigated in an attempt to elucidate some structure-function relationships of the protein. The circular dichroic spectrum of ferricytochrome c in the Soret region exhibited extrema at 417 and 402 nm, which were susceptible to the presence of alcohols in solution. In con...

A highly sensitive technique based on optical absorption using a single-mode, channel integrated optical waveguide is described for broad spectral band detection and analysis of heme-containing protein films at a glass/water interface. Fabrication steps and device characteristics of optical waveguides suitable for operation in the wavelength range of 400 - 650 nm are described. Experimental res...

We combined femtosecond (fs) VIS pump-IR probe spectroscopy with fs VIS pump-supercontinuum probe spectroscopy to characterize the photoreaction of the hexacoordinated Al(tpfc-Br8)(py)2 in a comprehensive way. Upon fs excitation at ∼400 nm in the Soret band, the excitation energy relaxes with a time constant of (250 ± 80) fs to the S2 and S1 electronic excited states. This is evident from the r...

Interactions of azide ion with bovine heart cytochrome c oxidase (CcO) at five redox levels (IV) to (0), obtained by zero to four electron reduction of fully oxidized enzyme CcO(IV), were monitored by infrared and visible/Soret spectra. Partially reduced CcO gave three azide asymmetric stretch band at 2040, 2016, and 2004 cm-1 for CcO(III)N3 and two at 2040 and 2016 cm-1 for CcO(II)N3 and CcO(I...

The procedure of Dietz et al. (Clin. Chem. 19: 1309-1313, 1973) for plasma cholinesterase (EC 3.1.1.7) gives a background absorbance of 1.4 A when extended to erythrocyte cholinesterase (EC 3.1.1.8) measurement, because the peak absorbance of the reaction product, 5-thionitrobenzoate, coincides with the hemoglobin Soret band at 410 nm. Consequently, the precision of erythrocyte cholinesterase m...

A model is proposed for chromophore optical spectra in solids over a wide range of temperatures and pressures. Inhomogeneous band shapes and their pressure dependence, as well as baric shift coefficients of spectral lines, selected by the frequency, were derived using Lennard-Jones potentials of the ground and excited states. Quadratic electron-phonon coupling constants, describing the thermal ...

The exact structure of the type-I11 isomer penta-, hexaand hepta-carboxylic porphyrinogens that are involved as intermediates in the conversion of uroporphyrinogen I11 into coproporphyrinogen 111 have been elucidated (Jackson et al., 1976). The corresponding porphyrins are to be found in the urine of patients suffering from the porphyrin-overproduction diseases symptomatic porphyria and congeni...