نتایج جستجو برای: spectrofluorometry
تعداد نتایج: 199 فیلتر نتایج به سال:
Background: The lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. The purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. In addition, correlation of lipid peroxidation with mot...
BACKGROUND Tumor necrosis factor (TNF)-alpha has been implicated in the pathogenesis of heart failure and ischemia-reperfusion injury. Effects of TNF-alpha are initiated by membrane receptors coupled to sphingomyelinase signaling and include altered metabolism and calcium cycling, contractile dysfunction, and cell death. We postulate that pressure-overload hypertrophy results in increased myoca...
BACKGROUND Mitochondrial changes that characterize the heart after anesthetic preconditioning (APC) or the mechanisms by which mitochondrial triggering factors lead to protection are unknown. This study hypothesized that generation of reactive oxygen species (ROS) during APC is required to initiate the mitochondrial protective effects, and that APC leads to improved mitochondrial electron trans...
Rutten, Michael J., Kathy D. Bacon, Katie L. Marlink, Mark Stoney, Camie L. Meichsner, Fred P. Lee, Susan A. Hobson, Karin D. Rodland, Brett C. Sheppard, Donald D. Trunkey, Karen E. Deveney, and Clifford W. Deveney. Identification of a functional Ca21-sensing receptor in normal human gastric mucous epithelial cells. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G662–G670, 1999.—The pur...
Lipofuscin accumulation has been observed in a number of neurodegenerative diseases. We recently found that autofluorescent particles also occur in the aged human optic nerve. In this study we sought to determine the nature of these particles and their correlation with aging, age-related macular degeneration (AMD) and primary open angle glaucoma (POAG). Groups of eight optic nerves from patient...
To clarify the effect of concentration polarization of oxidative modification of low-density lipoproteins (ox-LDLs) on human smooth muscle cells (SMCs), the proliferation, ox-LDL uptake and apoptosis with SMCs cultured on permeable (the permeable group) or non-permeable membranes (the non-permeable group) were analysed by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay...
To determine the direct effect of leptin on adipose tissue apoptosis in vitro using rat adipose-derived stem cells (ADSCs), we isolated the ADSCs of rat epididymis adipose tissue by collagenase digestion, filtration, and subsequent centrifugation. Cell cultures with or without leptin (10(-9) mol/L, 10(-8) mol/L, 10(-7) mol/L and 10(-6) mol/L) were incubated for different time. We examined the c...
OBJECTIVES The objective of this study was to determine the contribution of the CmeABC efflux pump to the emergence of fluoroquinolone (FQ)-resistant mutants in Campylobacter jejuni under various levels of selection pressure. METHODS The frequency of emergence of ciprofloxacin-resistant mutants was measured in wild-type C. jejuni NCTC 11168 and its isogenic cmeB mutant and cmeR mutant (overex...
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