Stable isotope paleoclimatology of the earliest Eocene using kimberlite-hosted mummified wood from the Canadian Subarctic B. A. Hook, J. Halfar, Z. Gedalof, J. Bollmann, and D. Schulze Department of Earth Sciences, University of Toronto, Toronto, ON M5S 3B1, Canada Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ON L5L 1C6, Canada Department of Geog...

In this study, we synthesized Herceptin conjugated magnetic nanoparticles (HMNs) as an alternative probe to discover the levels of HER2 (Human epidermal growth factor receptor-2) in the surface of cells. These nanoparticles can be used by magnetic resonance imaging (MRI) (non-invasive methods) for screening the patients with HER2 positive or negative tumors. Dextran coated iron oxide nanopartic...

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...

This paper deals with a strategic issue in the stable marriage model with complete preference lists (i.e., a preference list of an agent is a permutation of all the members of the opposite sex). Given complete preference lists of nmen over n women, and a marriage μ, we consider the problem for finding preference lists of n women over n men such that the men-proposing deferred acceptance algorit...

The stable marriage problem is that of matching n men and n women, each of whom has ranked the members of the opposite sex in order of preference, so that no unmatched couple both prefer each other to their partners under the matching. At least one stable matching exists for every stable marriage instance, and efficient algorithms for finding such a matching are well known. The stable roommates...

Eastern boundary currents are often described as 'wasp-waist' ecosystems in which one or few mid-level forage species support a high diversity of larger predators that are highly susceptible to fluctuations in prey biomass. The assumption of wasp-waist control has not been empirically tested in all such ecosystems. This study used stable isotope analysis to test the hypothesis of wasp-waist con...

Background: Microtubules (MT) are important components of cell cytoskeleton and play key roles in cell motility mitosis and meiosis. They are also the targets of several anticancer agents which indicating their importance in maintaining cell viability. Microtubular reorganization contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic...

A rapid isocratic method of high performance liquid chromatography system (HPLC) with a glassy carbon working electrode of electrochemical detector is set up for quantitative detection of  trace amount of nitrite ion (NO2¯) in aqueous protein containing cell lysate, cell media, plasma, serum, urine and other body fluids. The solid extraction  reversed phase cartridges ...

The mammalian cell lines HEK293 and CHO have become important expression hosts in structural biology. Generating stable mammalian cell lines remains essential for studying the function and structure of recombinant proteins, despite the emergence of highly efficient transient transfection protocols. Production with stable cell lines can be scaled up easily and high volumetric product yield can b...