We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucleases by monitoring DNA cleavage events with deep sequencing. The endonuclease specificities revea...

background: this study testing the effectiveness ,toxicity and tolereability of new chemotherapeutic regimen (consist of irinotecan 5-fu and leucovorin) in locally advanced and metastatic gastric cancer. patients and methods: patients with locally advanced gstric cancer (atleast stage 3)or metastatic gastric cancer that no treated previously with chemotherapeutic agents entered onto this study....

[1] We expand the theory of critically tapered Coulomb wedge for accretionary prisms by considering stress changes in subduction earthquake cycles. Building on the Coulomb plasticity of the classical theory, we assume an elastic–perfectly Coulomb plastic rheology and derive exact stress solutions for stable and critical wedges. The new theory postulates that the actively deforming, most seaward...

Multiplicative relations in the cohomology ring of a manifold impose constraints upon its stable systoles. Given a compact Riemannian manifold (X, g), its real homology H∗(X, R) is naturally endowed with the stable norm. Briefly, if h ∈ Hk(X, R) then the stable norm of h is the infimum of the Riemannian kvolumes of real cycles representing h. The stable k-systole is the minimum of the stable no...

Mobile agents operating in networked environments face threats from other agents as well as from the hosts (i.e., network sites) they visit. A black hole is a harmful host that destroys incoming agents without leaving any trace. To determine the location of such a harmful host is a dangerous but crucial task, called black hole search. The most important parameter for a solution strategy is the ...

In this paper, we prove the global asymptotic stability of a class of mass action futile cycle networks which includes a model of processive multisite phosphorylation networks. The proof consists of two parts. In the first part, we prove that there is a unique equilibrium in every positive compatibility class. In the second part, we make use of a piecewise linear in rates Lyapunov function in o...

We analyze the computational complexity of various rolling cube puzzles.

A partial orthomorphism of a group G (with additive notation) is an injection π : S → G for some S ⊆ G such that π(x) − x 6= π(y) − y for all distinct x, y ∈ S. We refer to |S| as the size of π, and if S = G, then π is an orthomorphism. Despite receiving a fair amount of attention in the research literature, many basic questions remain concerning the number of orthomorphisms of a given group, a...

Research so far has provided little evidence that benthic biogeochemical cycling is affected by ocean acidification under realistic climate change scenarios. We measured nutrient exchange and sediment community oxygen consumption (SCOC) rates to estimate nitrification in natural coastal permeable and fine sandy sediments under pre-phytoplankton bloom and bloom conditions. Ocean acidification, a...

Within last century the study of cycles of twodimensional quadratic systems was stimulated by 16th Hilbert’s problem and its different variants [Hilbert, 1902; Lloyd, 1988; Blows & Perko, 1994; Ilyashenko, 2002; Chen & Wang, 1979; Shi, 1980]. The development of asymptotical methods for investigation of cycles of quadratic systems is facilitated by the fractionally-quadratic transformations, whi...