This study tests the representativeness of groundwater recharge estimates through the chloride mass balance (CMB) method in a tropical environment. The representativeness of recharge estimates using this methodology is tested using evaporation estimates from isotope data, the general spatial distribution of the potential field, and the topographical variations in the area. This study suggests t...

Although bulk protein turnover has been measured with the use of stable isotope labeled tracers for over half a century, it is only recently that the same approach has become applicable to the level of the proteome, permitting analysis of the turnover of many proteins instead of single proteins or an aggregated protein pool. The optimal experimental design for turnover studies is dependent on t...

Calcified cyanobacterial microfossils are common in carbonate environments through most of the Phanerozoic, but are absent from the marine rock record over the past 65 Myr. There has been long-standing debate on the factors controlling the formation and temporal distribution of these fossils, fostered by the lack of a suitable modern analog. We describe calcified cyanobacteria filaments in a mo...

To minimize confusion in the expression of measurement results of stable isotope and gas-ratio measurements, recommendations based on publications of the Commission on Isotopic Abundances and Atomic Weights of the International Union of Pure and Applied Chemistry (IUPAC) are presented. Whenever feasible, entries are consistent with the Système International d'Unités, the SI (known in English as...

A stable isotope study was carried out seasonally in three coastal ponds (Marinello system, Italy) affected by different gull guano input to investigate the effect of nutrient subsidies on food web structure and dynamics. A marked 15N enrichment occurred in the pond receiving the highest guano input, indicating that gull-derived fertilization (guanotrophication) had a strong localised effect an...

Diazo groups are found in a range of natural products that possess potent biological activities. Despite longstanding interest in these metabolites, diazo group biosynthesis is not well understood, in part because of difficulties in identifying specific genes linked to diazo formation. Here we describe the discovery of the gene cluster that produces the o-diazoquinone natural product cremeomyci...

Department of Zoology and Physiology, University of Wyoming, 1000 East University Avenue, Department 3166, Laramie, WY 82071, USA (SDN) Department of Ecology and Evolutionary Biology, University of California–Santa Cruz, Santa Cruz, CA 95064, USA (JDY) Center for Isotope Geochemistry, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS 70A-4418, Berkeley, CA 94720, USA (PVW) United Stat...

Phosphatidylcholine (PC) is a very abundant membrane lipid in most eukaryotes, including yeast. The molecular species profile of PC, i.e. the ensemble of PC molecules with acyl chains differing in number of carbon atoms and double bonds, is important for membrane function. Pathways of PC synthesis and turnover maintain PC homoeostasis and determine the molecular species profile of PC. Studies a...

We demonstrate the first successful application of infrared laser spectrometry to the accurate, simultaneous determination of the relative H/H, O/O, and O/O isotope abundance ratios in natural water. The method uses a narrow line width color center laser to record the direct absorption spectrum of low-pressure gas-phase water samples (presently 10 μl liquid) in the 3 μm spectral region. The pre...

Maternal effects are widespread in ecology and can alter the dynamics of a population. We investigated the impact of maternal foraging strategies on offspring weaning mass--a proxy of maternal foraging success and of offspring survival--in southern elephant seals on îles Kerguelen. Using 4 years of data, we modelled pup weaning mass as a two-component mixture and used blood stable isotope value...