Human serum can be depleted of insulin and growth hormone by treatment with dextran-coated charcoal or by dialysis, or both. Inclusion of such hormone-depleted serum in the standard curve of a double-antibody radioimmunoassay for immunoreactive insulin more nearly mimics the conditions under which an unknown human serum sample is assayed. Compared to the usual standard curve in which serum albu...

Introduction: Gene expression and purification of luciferases from the firefly, Lampyris turkestanicus, and optimization of cellular ATP measurements were performed. Methods: cDNA encoding luciferases from Lampyris turkestanicus was transferred from pQE30 vector into pET28a expression vector and pLtu28 was built. Newly constructed vector was expressed in E. coli XL1 Blue and the recombinant l...

introduction: quantification of epstein - barr virus (ebv) in peripheral blood mononuclear cells (pbmnc) of allogenic bone marrow transplant (bmt) recipients is important because ebv-associated posttransplant lymphoproliferative disease (ptld) can occur after transplantation due to immunosup-pression therapy. methods: to this end we chose real-time pcr using taqman probe. for the standard curve...

a flow injection system is reported for the determination of fluoride based on its effects on the fluorescence intensity of al-morin complex at ph 3.0 in ethanol-warn mixture. the effect of important parameters such as excitation and emission wavelengths, ph, reagents concenkations, flow rate of the carrier stream, sample injection volume, time of measurepent and interferences are reported. cat...

this study describes a new simple, sensitive and selective catalytic kinetic spectrophotometricmethod for the determination of vanadium (v). the method is based upon the catalytic effect ofvanadium (v) on the oxidation of carminic acid by bromate in sulfuric acid media. the reaction wasfollowed spectrophotometrically by measuring the decrease in absorbance at 490 nm and thedependence of sensiti...