Cephamycin C is a β-lactam antibiotic used as a raw material in several commercial antibiotics. The production and purification process of this antibiotic requires a fast, simple and accurate method to quantify it. In this paper, it was developed a high-performance liquid chromatography method to determine cephamycin C concentration, in order to offer an option for the traditional, but laboriou...

We evaluated the effect of new beta-lactam antibiotics (azlocillin, mezlocillin, piperacillin, cefotaxime, moxalactam, and cefoperazone) on assays for aminoglycosides and vancomycin. These antibiotics produced no interference in an immunoassay for gentamicin and tobramycin. The new penicillins produced no interference in a bioassay of amikacin and vancomycin with penicillinase incorporated into...

Aliquots (0.1 mL) of whole-blood pools prepared to contain various concentrations of phenylalanine were applied to filter-paper collection cards, dried, and stored in sealed bags. We measured the phenylalanine content of the dried blood spots by bioassay, fluorometry, and "high-performance" liquid chromatography, and found that the concentrations remained constant for two years when samples wer...

background and objectives: due to the importance of finding of new antibacterial agents, the antibacterial properties of prosopis cineraria aerial parts were investigated using a bioassay guided fractionation scheme. materials and methods: the organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. the mics of fractions were deter...