conclusions in iran, hsct has been successfully adapted in routine clinical care. recently, new methods such as double cord blood and haploidentical transplantation have been used to treat many life-threatening diseases. results about 78.2% of the patients (2530 of 3237) remained alive between one to 211 months after stem cell transplantation. nearly, 21.8% (707) of our patients died after stem...

objective(s):the mesenchymal stem cells (mscs) have been introduced as appropriate cells for tissue engineering and medical applications. some studies have shown that topography of materials especially physical surface characteristics and particles size could enhance adhesion and proliferation of osteoblasts. in the present research, we studied the distinction effect of 30 and 60 μg/ml of zinc ...

objective: this study presents a simple method for isolation, expansion and purification of neonatal mouse spermatogonial stem cells. methods: we used enzymatic digestion to isolate a cell suspension of spermatogonia and sertoli cells from neonatal 2-day-old mice. the cells were cultured in dmem/f12 that contained 10% serum for two weeks. sertoli and spermatogonia cell characteristics were conf...

objective(s) some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. in the present study proliferation capacity and aging of such cells were explored. materials and methods adherent cells were isolated from the collagenase digests of adipose tiss...

Introduction  Insulin-like growth factor I (IGF-I) which is produced in abundance in the normal adult liver, is deeply involved in hepatocyte survival, growth, and differentiation during liver development. IGF-I plays the roles via the receptor (IGF-IR) signaling pathway. IGF-IR unlike IGF-I is expressed strongly in the developing liver, but much more weakly in adults. Objective:  We hypothesi...

Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...

mesenchymal stem cells (mscs),as the major stem cells for cell therapy, have been studied from different aspects in pre-clinical and clinical settings for more than a decade. these cells modulate the immune system (humoral and cellular immune responses) in vitro by producing soluble factors (anti-inflammatory molecules) and/or making cell-cell contacts. hence they could be used in regenerative ...

according to significant improvements in the tissue engineering field over the past several years, lung tissue cells have recently attracted more attention due to the high prevalence and diversity in related diseases. however, selection of an appropriate cell type, screening of suitable conditions for growth and proliferation, as well as subsequent implantation into the body to repair and regen...