A sex-steroid binding protein (SBP) that binds both testosterone and estradiol-17 beta was identified in the plasma and ovarian interstitial fluids of the spotted seatrout, Cynoscion nebulosus. Scatchard analysis revealed a single binding component with dissociation constants of 4.89 +/- 0.19 and 3.13 +/- 0.11 nM and binding capacities of 423 +/- 25 and 482 +/- 24 nM for testosterone and 17 bet...

1. A simple method for the extraction of 17-oxo steroid sulphates of plasma is described; glucosiduronates and orthophosphates are extracted, but to a smaller extent. 2. Four methods of analyses of the extracts are given and are relatively simple. Three of these are specific for steroid sulphates and two measure the sulphate conjugates directly. 3. Values for dehydroepiandrosterone sulphate and...

Exposure to phthalates in utero alters fetal rat testis gene expression and testosterone production, but much remains to be done to understand the mechanisms underlying the direct action of phthalate within the fetal testis. We aimed to investigate the direct mechanisms of action of mono-(2-ethylhexyl) phthalate (MEHP) on the rat fetal testis, focusing on Leydig cell steroidogenesis in particul...

Using the in vitro perfused rat testis, the effects of testosterone (T) on its own biosynthesis, and in particular on the inhibition of specific steroidogenic step(s) in the biosynthetic pathway from cholesterol to T, were examined. Rat testes perfused in vitro for 1 hour with medium containing 1.5 microM T secreted significantly less T than control testes in response to physiologic or maximal ...

Pregnenolone (PREG) and dehydroepiandrosterone (DHEA) have been reported to improve memory in aged rodents. In brain, these neurosteroids are transformed predominantly into 7alpha-hydroxylated metabolites by the cytochrome P450-7B1 (CYP7B). The biological role of steroid B-ring hydroxylation is unclear. It has been proposed to generate bioactive derivatives that enhance cognition, immune, and o...

myelography is a diagnostic procedure to indicate spinal defects. after the inception of new means of spinal cord imaging, use of myelography has been limited. since there are contraindications for other modalities in some patients, we have to use myelography. the most common complication of myelography is post myelography headache (pmh). many methods have been proposed to alleviate the pain. i...

In an attempt to assess histophysiological implication of the follicular compartment of the bovine ovary in steroid hormone formation and the effect of human chorionic gonadotropin (hCG) in vitro on follicular steroidogenesis, minces of follicular tissues from non-gravid bovine ovaries were incubated with radioactive testosterone or acetate in the presence and absence of hCG. Significant amount...

introduction: there are controversies regarding treatment of peritonsillar abscess as a common infection of head and neck, especially for prescription of corticosteroids. according to review of articles, use of corticosteroids in management of this abscess is increasing, but more controlled and comparative studies are needed. in this clinical trial study, we evaluated corticosteroid as an adjut...

Chronic obstructive pulmonary disease (COPD) is characterized by reduced lung function associated with increased local and systemic inflammatory markers, such as TNFα and IL-1β. Glucocorticoids are used to treat this chronic disease, however their efficacy is low and new drugs are very much required. 17-oxo-DHA is a cyclooxygenase-2-dependent, electrophilic, α,β-unsaturated keto-derivative of d...