A major challenge to economically produce ethanol from lignocellulosic biomass is to achieve industrially relevant ethanol titers (>50 g L) to control operating and capital costs for downstream ethanol operations while maintaining high ethanol yields. However, due to reduced fermentation effectiveness at high biomass solids loadings, excessive amounts of enzymes are typically required to obtain...

Effect of Poultry Litter Biochar on Saccharomyces cerevisiae Growth and Ethanol Production from Steam-exploded Poplar and Corn Stover

a complete diet (total mixed ration) with sorghum stover (40%) as sole roughage source was prepared and offered to animals under two forms: as mash or as expanded-extruded pellets (eep). these two complete diets were compared with a conventional diet (ad libitum chopped sorghum stover fed separately with concentrate mixture to meet 80% of protein requirements). these three diets were randomly a...

Genomewide selection (GWS) is markerassisted selection without identifying markers with signifi cant effects. Our previous work with the intermated B73 × Mo17 maize (Zea mays L.) population revealed signifi cant variation for grain yield and stover-quality traits important for cellulosic ethanol production. Our objectives were to determine (i) if realized gains from selection are larger with GW...

BACKGROUND Many different feedstocks are under consideration for the practical production of biofuels from lignocellulosic materials. The best choice under any particular combination of economic, agronomic, and environmental conditions depends on multiple factors. The use of old fields, restored prairie, or marginal lands to grow biofuel feedstocks offers several potential benefits including mi...

In Exp. 1, 4 ruminally and duodenally cannulated beef steers (444.0 +/- 9.8 kg) were used in a 4 x 4 Latin square with a 2 x 2 factorial treatment arrangement to evaluate the effects of forage type (alfalfa or corn stover) and concentrated separator byproduct (CSB) supplementation (0 or 10% of dietary DM) on intake, site of digestion, and microbial efficiency. In Exp. 2, 5 wethers (44 +/- 1.5 k...

The considerably low β-glucosidase activity : FPA ratio of 0.5-0.6 represented by the enzyme complex of Trichoderma reesei RUT C30 results in poor hydrolysis potential. To overcome this problem culture conditions were developed to enhance β-glucosidase production. Mandels’ medium supplemented with tris-maleate buffer proved to be efficient for cellulase production with elevated β-glucosidase ac...

Trichoderma reesei expresses a large number of enzymes involved in lignocellulose hydrolysis and the mechanism of how these enzymes work together is too complex to study by traditional methods, for example, by spiking with single enzymes and monitoring hydrolysis performance. In this study, a multivariate approach, partial least squares regression, was used to see whether it could help explain ...

BACKGROUND Microalgae are widely studied for biofuel production. Nevertheless, harvesting step of biomass is still a critical challenge. Bioflocculants have been applied in numerous applications including the low-cost harvest of microalgae. A major bottleneck for commercial application of bioflocculant is its high production cost. Lignocellulosic substrates are abundantly available. Hence, the ...

Corn stover harvest and transport cost functions were estimated for two harvest operations for a proposed biomass-to-ethanol conversion facility located in southern Minnesota, USA. This work presents an alternative methodology to estimating corn stover quantities and harvest costs at the county level, taking into account county-specific yields, transportation distances, erosion constraints, mac...