Instructional effects of tracking could have serious consequences for students and important implications educational policy practice. This study aimed to find out whether during senior high school matters when choosing a program in college. For this purpose, descriptive design was employed address the problem study. On other hand, due some problems which cannot be addressed quantitatively, the...

In this paper, we present a detailed overview of StrandCast, an application-layer multicast protocol for stretch invariant applications. StrandCast’s distribution topology is a linear list of participating receivers, which we call a strand. We describe three benefits that StrandCast has over traditional application-layer multicast topologies. The first is low and constant stress levels. The sec...

This buffer was also reported incorrectly in the Protocol S1. The second sentence under the heading ‘‘Global Single or minimal number Cell RT-PCR (GSC RT-PCR)’’ on the second page should be: ‘‘For 100 lL of lysis buffer, we combined 76 lL of RNase free water, 20 lL of first strand buffer 5x (250 mM Tris-HCl [pH 8.3], 375 mM KCl, 15 mM MgCl2), 1 lL of Prime RNase inhibitor (Brinkmann, Westbury, ...

We introduce ppsim [], a software package for efficiently simulating population protocols, widely-studied subclass of chemical reaction networks (CRNs) in which all reactions have two reactants and products. Each step the dynamics involves picking uniform random pair from n molecules to collide (potentially null) reaction. In recent breakthrough, Berenbrink, Hammer, Kaaser, Meyer, Penschuck, Tr...

BACKGROUND The envelope (E) protein of dengue virus (DENV) is the major target of neutralizing antibodies and vaccine development. While previous studies on domain III or domain I/II alone have reported several epitopes of monoclonal antibodies (mAbs) against DENV E protein, the possibility of interdomain epitopes and the relationship between epitopes and neutralizing potency remain largely une...

The controlled fragmentation of chromosomes by DNA double-strand breaks (DSBs) initiates meiotic recombination, which is essential for meiotic chromosome segregation in most eukaryotes. This chapter describes a straightforward microarray-based approach to measure the genome-wide distribution of meiotic DSBs by detecting the single-stranded DNA (ssDNA) that transiently accumulates at DSB sites d...

The generation of precise alterations to the genome using CRISPR requires the combination of CRISPR and a donor construct containing homology to the target site. A double-strand break is first generated at the target locus using CRISPR. It is then repaired using the endogenous homologous recombination (HR) pathway. When a donor construct is provided, it can be used as a template for HR repair a...

We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplif...

We report a real-time DNA detection method that utilizes single-strand DNA-modified nanoparticle probes and micropatterned chemoresponsive diffraction gratings interrogated simultaneously at multiple laser wavelengths. The surface-bound nanoparticle probe based assay with the chemoresponsive diffraction grating signal transduction scheme results in an experimentally simple DNA detection protoco...