A new developmental mutant of Myxococcus xanthus has been isolated by screening TnV insertion mutants for AMI-dependent development in submerged culture. This mutant (ER304) aggregated and sporulated on agar surfaces but required at least 3.8 micrograms of autocide AMI per ml for development in submerged cultures. Spore rescue of ER304 was obtained with the saturated, monounsaturated, and diuns...

PURPOSE To investigate the use of a multistep serum-free culture system in developing a conjunctival epithelial equivalent with improved in vitro and in vivo proliferative properties and to evaluate the effect of serum supplementation and culture conditions on the proliferative capacity of these cells. METHODS Conjunctival epithelial cells were cultivated on human amniotic membrane (HAM) in a...

The research was conducted to select the nitrogen sources and suitable light condition during Ganoderma lucidum submerged culture (isolated in Vietnam). results showed that organic is more than inorganic for growth synthesis of fungal polysaccharides. amounts incellular polysaccharide exopolysaccharide this strain highest when combined 7.5 g/L yeast extract 2.5 peptone. affected on size, shape ...

In the most cases of the practical fermentation processes with filamentous microorganisms, direct monitoring of the cell morphology and biomass distribution in the culture medium is not easy possible. Penicillium brevicompactum is one of the morphologically complex filamentous fungi with different structural forms. In this research, nutrient uptake and cell growth kinetics was investigated comp...