Here, we show a novel molecular mechanism promoted by the DEAD-box RNA helicase DDX3 for translation of the HIV-1 genomic RNA. This occurs through the adenosine triphosphate-dependent formation of a translation initiation complex that is assembled at the 5' m(7)GTP cap of the HIV-1 mRNA. This is due to the property of DDX3 to substitute for the initiation factor eIF4E in the binding of the HIV-...

BACKGROUND A tube feeding decision aid designed at the Ottawa Health Research Institute was specifically created for substitute decision-makers who must decide whether to allow placement of a percutaneous endoscopic gastrostomy (PEG) tube in a cognitively impaired older person. We developed a Japanese version and found that the decision aid promoted the decision-making process of substitute dec...

BACKGROUND A phenylalanine-free amino acid based protein substitute is necessary to provide the major source of protein in phenylketonuria (PKU). Protein substitutes in PKU are usually given as drinks. These are unpalatable and compliance is often poor. Tablets containing a suitable mixture of phenylalanine-free amino acids (Aminogran Food Supplement, UCB) are now available. AIMS To compare t...

Objectives After reading this article, the reader will be able to:  Briefly discuss the significant milestones in the search for a substitute for human blood.  List several factors driving the search for a blood substitute.  List the characteristics of an ideal blood substitute.  Name and explain the four phases of clinical trials required before a drug can be approved by the Food and Drug ...

Lack of cytocompatibility in bone substitutes impairs healing in surrounding bone. Adverse biological events around biomaterials may be associated with oxidative stress. We hypothesized that a clinically used inorganic bone substitute is cytotoxic to osteoblasts due to oxidative stress and that N-acetyl cysteine (NAC), an antioxidant amino acid derivative, would detoxify such material. Only 20%...

The plastid ClpPR protease complex in Arabidopsis thaliana consists of five catalytic ClpP and four noncatalytic ClpR subunits. An extensive analysis of the CLPR family and CLPP5 is presented to address this complexity. Null alleles for CLPR2 and CLPR4 showed delayed embryogenesis and albino embryos, with seedling development blocked in the cotyledon stage; this developmental block was overcome...

Although the boundary elements of the Drosophila Bithorax complex (BX-C) have properties similar to chromatin insulators, genetic substitution experiments have demonstrated that these elements do more than simply insulate adjacent cis-regulatory domains. Many BX-C boundaries lie between enhancers and their target promoter, and must modulate their activity to allow distal enhancers to communicat...

There have been several studies on the kinetics of . oxygen uptake (VO2) responding to impulse work (Bakker et al., 1980; Hughson et al., 1988; Swanson, 1990; Whipp and Ward, 1991), and it has been demonstrated . that VO2 is composed of two components (phase I and phase II). With regard to these components, Hughson et al. (1988) have suggested that phase I reflects an immediate increase in pulm...

Blood and stripped hemoglobin from Nelore cattle individuals were submitted to oxygen equilibrium experiments in both gasometric and spectrophotometric methods. No difference was detected in oxygen affinity and Bohr effect among Hb A, Hb B and Hb AB in experiments with both blood and stripped hemoglobin, in the presence and absence of the chloride ion. However, n values (Hill plots) were higher...

14 Regulatory, safety, and environmental issues have prompted the development of aqueous 15 enzymatic extraction (AEE) for extracting components from oil-bearing materials. The 16 emulsion resulting from AEE requires de-emulsification to separate the oil; when enzymes 17 are used for this purpose, the method is known as aqueous enzymatic emulsion de18 emulsification (AEED). In general, enzyme a...