نتایج جستجو برای: suppression subtractive hybridization

تعداد نتایج: 160708  

Journal: :BioTechniques 1997
H Jin X Cheng L Diatchenko P D Siebert C C Huang

We have developed a new strategy for differential screening of genes that are expressed in two or more tissues, and have used it to identify genes that are preferentially expressed in both testis and ovary. In this approach, testis-specific cDNAs were first generated using the suppression subtractive hybridization technique, cloned and arrayed in microplates. The inserts from the subtracted tes...

2012
Hongchun Xiong Takanori Kobayashi Yusuke Kakei Takeshi Senoura Mikio Nakazono Hirokazu Takahashi Hiromi Nakanishi Hongyun Shen Penggen Duan Xiaotong Guo Naoko K. Nishizawa Yuanmei Zuo

Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to divalent metal transporters of the natural resistance-associated macrophage protein (NRAMP) gene fami...

2016
YuDong Liu Li Zhang LiJing Chen Hui Ma YanYe Ruan Tao Xu ChuanQiang Xu Yi He MingFang Qi

Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5' UTR and 3' UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). A substrate reactio...

2013
Tianxiu Zhong Chen Zhu Huiming Zeng Liebao Han

Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtractive hybridization technique was used to investigate gene expression during asexual reproducti...

Journal: :The Journal of Experimental Medicine 2004
Jee-Youn Kim Hyun-Jong Ahn Jong-Hoon Ryu Kyoungho Suk Jae-Hoon Park

Hypoxia is a common cause of cell death and is implicated in many disease processes including stroke and chronic degenerative disorders. In response to hypoxia, cells express a variety of genes, which allow adaptation to altered metabolic demands, decreased oxygen demands, and the removal of irreversibly damaged cells. Using polymerase chain reaction-based suppression subtractive hybridization ...

Journal: :American journal of respiratory cell and molecular biology 2004
Katherine Dahlin Edward M Mager Lennell Allen Zachary Tigue Lee Goodglick Madhuri Wadehra Leland Dobbs

Although approximately 98% of the internal surface area of the lung is lined by alveolar type I cells, little is known about the functions of this cell type. Using freshly isolated rat type I and type II cells, we created a subtraction library by suppression subtractive hybridization to identify genes differentially expressed by type I cells. We identified twelve genes of known function that ar...

Journal: :Journal of medical microbiology 2002
Craig Winstanley

Comparison of DNA from virulent strains of bacterial pathogens with DNA from less virulent or avirulent close relatives allows the identification of those genomic regions that are present only in virulent strains. Such regions are often associated with pathogenicity islands (PIs) and their characterisation can lead to a greater understanding of the pathogenesis of infectious diseases. There is ...

2011
Torunn Forberg Augustine Arukwe

The commensal bacteria in the intestine play essential roles in the development and functionality of the host. To unravel the host^microbe interactions in Atlantic cod (Gadus morhua L.) larvae, we used two molecular approaches: (1) suppression subtractive hybridizationpolymerase chain reaction (SSH-PCR) to identify host gene responses and (2) expression analysis of selected genes reported to be...

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