The ultrastructure of neurosecretory cells in the paraventricular nucleus of the female rat in various stages of the estrous cycle was studied by electron microscopy. On account of the secretory cycle, the neurosecretory neurons were classified into four types, and the incidence of each type was counted throughout the estrous cycle. In proestrous afternoon, neurons at the phase of protein synth...

The present study reports for the first time the distribution of androgen receptor immunoreactivity (AR-ir) in the human hypothalamus of ten human subjects (five men and five women) ranging in age between 20 years and 39 years using the antibody PG21. Prolonged postmortem delay (72:00 hours) or fixation time (100 days) did not influence the AR-ir. In men, intense nuclear AR-ir was found in neur...

Whole cell patch-clamp recordings were obtained from supraoptic neurons to investigate the effects of histamine on excitatory postsynaptic currents evoked by electrical stimulation of areas around the posterior supraoptic nucleus. When cells were voltage-clamped at -70 mV, evoked excitatory postsynaptic currents had amplitudes of 88.4 +/- 9.6 pA and durations of 41.1 +/- 3.0 ms (mean +/- SE; n ...

To investigate the release profile of met-enkephalin, β-endorphin, and dynorphin-A in ruminants' CNS, goats were stimulated by electroacupuncture of 0, 2, 40, 60, 80, or 100 Hz for 30 min. The pain threshold was measured using potassium iontophoresis. The peptide levels were determined with SABC immunohistochemisty. The results showed that 60 Hz increased pain threshold by 91%; its increasing r...

P2X receptors are ATP-gated cationic channels composed of seven known subunits (P2X1-7) which are involved in different functions in neural tissue. The present study investigates the P2X5 receptor expression pattern in the mouse CNS using immunohistochemistry and in situ hybridization histochemistry. The specificity of the immunostaining has been verified by pre-absorption, Western blot and in ...

s: M.G. Spina, K. Langnaese, G.F. Orlando, S.A. Andrabi, T.F.W. Horn, J. Rivier, W. Vale, M.Engelmann and G. Wolf. Urocortin is coexpressed with neuronal nitric oxide synthase in thesupraoptic nucleus but not in the Edinger westphal nucleus of rats.1st Joint French-German NO Meeting, Strassbourg, France (1-4 October, 2003) M.G. Spina, K. Langnaese, G.F. Orlando, S.A. Andrabi, T.F.W....

The hypothalamic supraoptic nucleus (SON) is a core osmoregulatory control centre that deciphers information about the metabolic state of organism and orchestrates appropriate homeostatic (endocrine) allostatic (behavioural) responses. We have used RNA sequencing to describe polyadenylated transcriptome SON male Wistar Han rat. These data been mined generate comprehensive catalogues functional ...