We present numerical self-consistent field calculations in a two-gradient cylindrical coordinate system for a ~translationally restricted! quenched polyelectrolyte star which is electrostatically attracted to an oppositely charged surface with homogeneous surface charge density at a given ionic strength of a 1:1 electrolyte. The results prove that without any additional driving force for adsorp...

Electrostatic charge at the membrane surface has emerged as a crucial determinant of the localization and activation of many proteins containing polycationic domains in their amino acid sequence. The spatiotemporal regulation of surface charge, as well as the downstream effects of dysregulation of surface charge, may have a significant impact on many of the signaling molecules important to canc...

The utility of H NMR spectroscopy of headgroup deuterated amphiphiles as a tool for measuring charge density at the surface of lipid bilayer membranes and polymer colloids is described. Attention is given to current ideas regarding the physical origin of the spectral changes observed in the presence of surface charges, and to several novel applications of the technique including determination o...

Treatment of PS II particles with either 1 m NaCl or alkaline Tris (1 m , pH 8.4) caused a considerable decrease in the average net negative surface charge density, concomitant with de­ pletion of the extrinsic 17, 24 and 33 kDa proteins of the oxygen evolving complex from the membranes. The partial recovery of the values for surface charge in both NaCland Tris-treated membranes was registered ...

adsorption of bovine serum albumin (bsa) onto calcium hydroxyl apatite (ha), ca10po4(oh)2, has been studied by ultra violet (uv) spectroscopy. the adsorption isotherms of bsa onto the ha surface at 291.7 k and 303.2 k were satisfactorily presented by langmuir equation and the evaluated parameters are reported. the specific surface area of ha has been measured by the bet method. the obtained val...

Optimization of surface exposed charge-charge interactions in the native state has emerged as an effective means to enhance protein stability; but the effect of electrostatic interactions on the kinetics of protein folding is not well understood. To investigate the kinetic consequences of surface charge optimization, we characterized the folding kinetics of a Fyn SH3 domain variant containing f...