Solid lipid nanoparticles of atovaquone (ATQ-SLN) were prepared by high shearhomogenization method using tripalmitin, trilaurin, and Compritol 888 ATO as the lipidmatrices and Phospholipon 90H, Tween 80, and poloxamer 188 as the surfactants. Optimizationof the formulations was conducted using 6 sets of 24 full-factorial design based on fourindependent variables that were the number of homogeniz...

Solid lipid nanoparticles of atovaquone (ATQ-SLN) were prepared by high shearhomogenization method using tripalmitin, trilaurin, and Compritol 888 ATO as the lipidmatrices and Phospholipon 90H, Tween 80, and poloxamer 188 as the surfactants. Optimizationof the formulations was conducted using 6 sets of 24 full-factorial design based on fourindependent variables that were the number of homogeniz...

Intratracheal instillation of Tween causes a heterogeneous surfactant deactivation in the lung, with areas of unstable alveoli directly adjacent to normal stable alveoli. We employed in vivo video microscopy to directly assess alveolar stability in normal and surfactant-deactivated lung and tested our hypothesis that alveolar instability causes a mechanical injury, initiating an inflammatory re...

Intratracheal instillation of Tween causes a heterogeneous surfactant deactivation in the lung, with areas of unstable alveoli directly adjacent to normal stable alveoli. We employed in vivo video microscopy to directly assess alveolar stability in normal and surfactant-deactivated lung and tested our hypothesis that alveolar instability causes a mechanical injury, initiating an inflammatory re...

Polyurethane nanoparticles (NPs) are promising candidates for the controlled and targeted delivery of therapeutics in a variety of biomedical applications. In this work, a report is made of NPs produced by miniemulsion polymerization with isophorone diisocyanate (IPDI) and castor oil, glycerol, and poly(ethylene glycol) (PEG) with molar masses 400 and 1000 as monomers and Tween 80, Span 80 and ...

Palm-based lipid nanoparticle formulation loaded with griseofulvin was prepared by hot homogenisation and a solvent-free method. The optimum griseofulvin loaded lipid nanoparticles were prepared via stages of optimisation, such as high pressure homogenisation (HPH) parameters, lipid and surfactant screening and selection of suitable lipid to surfactant ratios; followed by photon correlation stu...

Reversed micellar two-phase extraction is a developing technique for protein separation. Introduction of an affinity ligand is considered to be an effective approach to increase the selectivity and capacity of reversed micelles. In this article, Cibacron Blue F3G-A (CB) as an affinity ligand was immobilized to reversed micelles composed of soybean lecithin by a two-phase reaction. The affinity ...

A simple sequential injection (SI) method is reported for the determination of the non-ionic surfactant polyoxyethylene sorbitan monoleate (Tween-80). The proposed method is based on a fluorescence enhancement of the probe 4',5'-dibromo-2',7'-dinitro fluorescein, disodium salt (Eosin B) in the presence of a surfactant. The procedure is optimized using the univariate method of optimization. The ...

The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitatio...