Hemoglobin Setif produces pseudosickling of red cells in vitro; the nature of the process and the conditions that "trigger" it are unknown. Studies of red cells, hemolysates, purified hemoglobin solutions, and artificial mixtures of Hb A and Setif suggest that pseudosickling is produced by intracellular crystallization of insoluble hemoglobin. Increased tonicity of the suspending medium accentu...

Dielectric measurements revealed that dormant spores of Bacillus cereus have extremely low conductivities at high frequencies (50 MHz) and so must contain remarkably low concentrations of mobile ions both within the core and in the surrounding integuments. Activation, germination, and outgrowth were all accompanied by increases in conductivity of the cells and their suspending medium, and this ...

Chloroplasts isolated from the siphonous green alga Caulerpa simpliciuscula (Turner) C.Ag. were shown to be resistant to dissolution by the nonionic detergent Teric-10 at concentrations as high as 0.3% (v/v) when treated at 0 C. There was little release of stromal enzymes under these conditions. These chloroplasts were disrupted by osmotic shock as shown by measurement of the release of both gl...

1. Rat and rabbit erythrocyte plasma-membrane proteins were solubilized with decanoyl-N-methylglucamide and reconstituted into liposomes. The procedure includes detergent removal by gel filtration, followed by a freeze-thaw step. 2. The rate of [1-14C]pyruvate uptake into these vesicles was inhibited by approx. 70% by alpha-cyano-4-hydroxycinnamate and p-chloromercuribenzenesulphonate. The exte...

Rat liver mitochondria accumulate protoporphyrin IX from the suspending medium into the inner membrane in parallel with the magnitude of the transmembrane K+ gradient (K+in/K+out). Only protoporphyrin IX taken up in parallel with the transmembrane K+ gradient is available for haem synthesis. Coproporphyrins (isomers I and III) are not taken up by the mitochondria. The results support the sugges...

A finite difference/front tracking method is used to study the motion of three-dimensional deformable drops suspended in plane Poiseuille flow at non-zero Reynolds numbers. A parallel version of the code was used to study the behavior of suspension on a reasonable grid resolution (grids). The viscosity and density of drops are assumed to be equal to that of the suspending medium. The effect of ...

In this paper we present a constitutive relation for predicting the rheology of short glass fibers suspended in a polymeric matrix. The performance of the model is assessed through its ability to predict the steady-state and transient shear rheology as well as qualitatively predict the fiber orientation distribution of a short glass fiber (0.5 mm, L/D < 30) filled polypropylene. In this approac...

We present a strategy tbr suspending recursive open conditions during planning. We also show conditions under which plans with suspended open conditions can be pruned. To make this suspension and pruning strategy efficient, we use an operator graph to analyze potential recursion before the planning process begins. This approach covers a broader range of recursive problems than the approaches of...

The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various combinations of sucrose, glutamate, and potassium phosphates. Suspending media containing glutamat...

Protein synthesis is knownl-14 to occur within mitochondria, and several components of their translational apparatus have been isolated and characterized.' 15-21 O'Brien and Kalf19' 20 found that ribosomes of rat liver mitochondria have a sedimentation coefficient of 55S, in contrast to the cytoplasmic (microsomal) 78S ribosomal monomeric units. Similarly, Rogers et al.21 reported that yeast mi...