Next-generation DNA sequencing (NGS) has broad applications in both research and clinical diagnostics and has specifically become an important platform for identifying mutations and variants from clinical samples. Though NGS is capable of very high levels of coverage even on complex genomes, it is still advantageous to reduce the complexity of samples and sequence smaller targeted regions – in ...

Triangular silver nanoplates, of different orientations, and other nanostructures were successfully synthesized using the Horizontal Vapor Phase Crystal (HVPC) Growth technique for antimicrobial purposes in this study. This finding demonstrates HVPC as an alternative and simple technique to synthesize ordered silver nanomaterials for antimicrobial study. Keyword —alternative method, antimicrobi...

Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a I 10-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize PPDK precursor polypeptide was taken up from the translation product mixture b...

The preparation of synthetic ribonucleic acid in which one of the nucleotide species is labeled with P32, makes possible studies on the distribution of the labeled nucleotide in the polynucleotide chain. Fig. 1 presents the structure of a polynucleotide in which labeled adenylic acid is randomly distributed. Hydrolysis of such a polymer with snake venom phosphodiesterase (Fig. 1A) will yield a ...

Large degradable dendrimers (MW > 30 kDA) were synthesized in a divergent manner utilizing a novel 1,3,5-triazaadamantane (TAA) monomer that can degrade under acidic conditions.

De novo synthesis of long double-stranded DNA constructs has a myriad of applications in biology and biological engineering. However, its widespread adoption has been hindered by high costs. Cost can be significantly reduced by using oligonucleotides synthesized on high-density DNA chips. However, most methods for using off-chip DNA for gene synthesis have failed to scale due to the high error ...

The design complexity of systems on a chip drives the need to reuse legacy or intellectual property cores, whose gate-level implementation details are unavailable. The core test depends on manufacturing technologies and changes permanently during a design lifecycle. In this paper we consider the impact of circuit realization on the fault coverage of the test set. We have performed various compr...

A non-classic approach in the synthesis and crystal growth within electrostricted water solutions of highly charged ionic species allowed us to obtain bright monocrystals of the zwitterionic tetracarboxylate compound Mo2(O2CC6H3(NH3)2)4Cl8 16H2O (1). The title compound tetrakis-( -3,5-diaminobenzoate)octachlorodimolybdenum(II,II)—aqua(1/16) (1) crystallizes in the P21/c monoclinic space group w...

Aiming at the static task scheduling problems in heterogeneous environment, a heuristic task scheduling algorithm named HCPPEFT is proposed. In task prioritizing phase, there are three levels of priority in the algorithm to choose task. First, the critical tasks have the highest priority, secondly the tasks with longer path to exit task will be selected, and then algorithm will choose tasks wit...