I am a relic of the last century, born in 1918, the last year of the first World War. At that time, there was no radio, no television, no commercial air travel. There was no welfare, no social security, no Medicare, and only the rich paid income taxes. Influenza, strep throat, pneumonia, tuberculosis, polio, and high blood pressure were deadly diseases. I was 11 when the stock market crashed an...

A turbidimetric method for inorganic sulfates in urine, based on the precipitation of barium sulfate, is adapted to the AutoAnalyzer. Coating of the flowcell with BaSO4is avoided by an inter-sample air rinsing. Sampling rate is 40/h. Inorganic urinary sulfate determinations are of interest for the evaluation of endogenous acid production (1) and in experimental evaluation of the acid-excreting ...

我々の確立したWaS-ALD50 法は，APTT 延長検体のクロスミキシングテスト判定法であり，APTT 試薬としてトロンボチェックAPTT-SLA (SLA: Sysmex) を使用した．今回，コアグピアAPTT-N (CP-N: 積水メディカル) を用いたWaS-ALD50 法の評価を行った．

1. Kessler, G., and Wolfman, M., An automated procedure for the simultaneous determination of calcium and phosphorus. Clin. Chem. 10, 686 (1964). 2. Gitelman, H. J., An improved automated procedure for the determination of calcium in biological specimens. Anal. Biochem. 18, 521 (1967). 3. Lott, J. A., and Herman, T. S., Increased AutoAnalyzer dialysis of calcium and magnesium in presence of pro...

With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the Auto...

The enzymatic method for the determination of ammonia with glutamate dehydrogenase has been adapted to the AutoAnalyzer. The automated method was applicable for NH(3) measurement in sheep rumen samples, swine gut fluid samples, and bovine plasma. Results were compared with those of the Conway diffusion method and the manual enzymatic method. The automated method allows 30 samples per h to be an...

A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2amino-2-methyl-l-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple m...

Methods are described for assaying the fluoride content of ashed samples of plant and animal tissues. Using the Hall method, submicrogram quantities of fluoride were measured and a new procedure was devised to measure fluoride in a 1 to 8 microgram range. Modification of the Weinstein procedure, in which an AutoAnalyzer is used, permitted rapid and semiautomated analysis of samples containing 2...

The stability of 18 chemical constituents of human serum was measured at various times and temperatures, the measurements being made with the Technicon AutoAnalyzer, by routine N-methods (Technicon laboratory method file number). Data are presented illustrating what happens to human serum under defined conditions of collection and storage. Analysis of the data indicates that under certain condi...

The optimum analytical Conditions with regard to two or more variables can be determined in an unambiguous way by use of the simplex method. The method is particularly applicable to problems involving interacting variables. We describe the application of the simplex technique to the optimization of two procedures on a continuous-flow analyzer (AutoAnalyzer) and to the optimization of chemical v...