The adenovirus (Ad) genome in virion exists in the form of the Ad core, which is a complex of Ad DNA and viral basic proteins. The Ad core supported only initiation and limited elongation in DNA replication in a cell-free system which was developed for DNA replication of naked Ad DNA. We found that addition of a cytoplasmic fraction from uninfected HeLa cells on the top of the system stimulated...

Chronic hepatitis B virus (HBV) infection remains an important global health problem, and may be difficult to manage in clinical practice. Nucleos(t)ide analogues (NAs) with a high barrier resistance (entecavir [ETV], tenofovir disoproxil fumarate [TDF] alafenamide [TAF]) are the most frequently used HBV treatments because of their long-term effectiveness tolerability. ETV less effective patien...

The basal transcription factor TFIID consists of the TATA-binding protein (TBP) and TBP-associated factors (TAFs). Yeast Taf67 is homologous to mammalian TAF(II)55. Using a yeast two-hybrid screen to identify proteins that interact with Taf67, we isolated Bromodomain factor 1 (Bdf1) and its homolog (Bdf2). The Bdf proteins are genetically redundant, as cells are inviable without at least one of...

The synthesis and the spectroscopic characterization of fluoride-alkoxides of niobium and tantalum in the highest oxidation state are reported. Suspensions of MF(5) (M = Nb, Ta) in a chlorinated solvent reacted with up to three equivalents of ROSiMe(3) (R = Me, Et, Ph) to afford polynuclear derivatives and variable amounts of FSiMe(3). Thus MF(4)(OR) (R = Et, Ph) and MF(3)(OR)(2) were obtained ...

Contents Software Productivity Consortium (Consortium) members applying the tools for both the requirement and design model-driven verification and validation are often required to certify their software with various certification authorities such as the Federal Aviation Administration (FAA) and Food and Drug Administration (FDA). These certifications require methods and supporting artifacts fo...

Emtricitabine (FTC) plus tenofovir alafenamide (TAF) has demonstrated efficacy and safety for pre-exposure prophylaxis (PrEP) to prevent HIV-1 infection. We measured the plasma PK of FTC, (TFV), TAF in a steady-state pharmacokinetic (PK) study bictegravir/FTC/TAF HIV-1-infected patients. Furthermore, validated liquid chromatography-tandem mass spectrometry was used measure intracellular TFV-dip...

Investigations into long-term geomagnetic variations provide useful information regarding paleomagnetic field behavior. In this study, we assess the latitudinal structure of paleosecular variation (PSV) and time-averaged (TAF) for Brunhes normal Matuyama reverse chrons, 0–10 Ma period, from an updated reviewed paleodirectional database spanning past 10 Myr. The new comprises 2,543 sites igneous...

Virtual agents are used by the military for extensive training of pilots. However, creating virtual agents with the appropriate behaviors is a lengthy and specialized process. We describe a new tool (TAF) to allow rapid creation of virtual adversarial agents by non-specialized personnel. In TAF, users can draw diagrams of agent behavior which are matched within a pre-existing knowledge base. By...

In this paper we present the method of lines for the numerical solution of a mathematical model for capillary formation in two space dimensions x,y. We study the tumor angiogenic factor (TAF) equation, and suppose there is no tumor source supplied to the system for simplicity. This method is an approach to the numerical solution of partial differential equations that involve a time variable t a...