Evidence in support of safety of a gene delivery procedure is essential toward gene therapy. Previous studies using the hydrodynamics-based procedure primarily focus on gene delivery efficiency or gene function analysis in mice. The current study focuses on an assessment of the safety of computer-controlled and liver-targeted hydrodynamic gene delivery in dogs as the first step toward hydrodyna...

The work demonstrates a judicious approach to achieve hepatocyte cell-targeted highly efficient gene delivery by utilizing a supramolecular complex of galactosylated cucurbituril and dextran-spermine conjugates.

Novel drug delivery system utilizing smart polymer to get significant and attracting changes in the targeting of drugs, increasing bioavailability enhancement patient compliance gene therapy. The scientific community tries mimic nature way that living organisms adopt their behavior as a function environmental conditions improve survival. In this sense, polymers offer materials respond numerous ...

Efforts to influence the biology of blood vessels by gene delivery have been hampered by a lack of targeting vectors specific for endothelial cells in diseased tissues. Here we show that a cationic nanoparticle (NP) coupled to an integrin alphavbeta3-targeting ligand can deliver genes selectively to angiogenic blood vessels in tumor-bearing mice. The therapeutic efficacy of this approach was te...

PURPOSE To evaluate the efficacy of a novel docetaxel derivative of deslorelin, a luteinizing hormone-releasing hormone (LHRH) agonist, and its combination in vivo with RGD peptide conjugated nanoparticles encapsulating an antiangiogenic, anti-vascular endothelial growth factor (VEGF) intraceptor (Flt23k; RGD-Flt23k-NP) in H1299 lung cancer cells and/or xenografts in athymic nude BALB/c mice. ...

Clohisy, DR; +Ramnaraine, ML; Lynch, CM; Pan, W; Donahue, JM +Department of Orthopedic Surgery, University of Minnesota, Minneapolis, MN [email protected] INTRODUCTON: One of the most significant advances in understanding the pathophysiology of bone metastases has been the discovery that intense osteoclastogenesis occurs at sites of tumor (1), a feature common to both primary bone sarcomas and m...

Pulmonary arterial hypertension (PAH) remains a fatal disease despite modern pharmacotherapy. Mutations in the gene for bone morphogenetic protein receptor type II (BMPR2) lead to reduced BMPR2 expression, which is causally linked to PAH. BMPR2 is predominantly expressed on pulmonary endothelium and has complex interactions with transforming growth factor (TGF)-β signalling mechanisms. Our obje...

The major obstacle to developing siRNA delivery is their extracellular and intracellular barriers. Herein, a humanized anti-EGFR monoclonal antibody h-R3 was developed to modify the self-assembled binary complexes (dendriplexes) of PAMAM and siRNA via electrostatic interactions, and two common ligands HSA and EGF were used as a control. Compared to dendriplexes, h-R3/EGF/HSA-dendriplexes showed...