background: bilateral ectopic pregnancy is a rare condition and is divided in two subgroups, primary and secondary, based on history of assisted reproductive technology. case presentation: a 30 year old primigravid woman with history of infertility and ovulation induction presented to a hospital in kashan in year 2013. she had vaginal bleeding, abdominal pain and ultrasound findings suggested e...

introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...

Background: The variable numbers of tandem-repeat (VNTR) alleles at the phenylalanine hydroxylase (PAH) gene have been used in carrier detection and prenatal diagnosis in phenylketonuria families. This study was carried out to analyze VNTR alleles at the PAH gene in Iranian Azeri Turkish population. Methods: In this study, 200 alleles from general population were studied by PCR. Results: The fr...

introduction: mucous retention cysts are a subtype of intracordal vocal cysts that may occur spontaneously or may be associated with poor vocal hygiene, and which require optimal treatment. the objective of this study was to present a new laser-assisted microsurgery technique for treating intracordal mucous retention cysts and to describe the final outcomes.   materials and methods: in this pro...

This article presents a technique for the determination of the relative misregistration between two interferometric SAR images. The proposed technique is based on the spectral properties of the complex SAR signal. Unlike conventional coregistration methods, the proposed technique does not need any interpolation nor cross-correlation procedures and also no coherence or fringe optimization must b...

Over the past decade, the human identity testing community has settled on a set of core short tandem repeat (STR) loci that are widely used for DNA typing applications. A variety of commercial kits enable robust amplification of these core STR loci. A brief history is presented regarding the selection of core autosomal and Y-chromosomal STR markers. The physical location of each STR locus in th...

The use of genomic DNA-based techniques in ecological and evolutionary studies has been limited by the availability of suitable probes for species of interest due to the technical difficulty of isolating and applying such probes. We have developed a simple technique that directs polymerase chain reaction (PCR) amplification to regions rich in variable number of tandem repeats (VNTRs). By using ...

In a l l adenovlruses (AV) studied to date the DNA possesses a unique inverted terminal repeat (ITR) of the form abc . . . o'b a .Although the repeat sequences are t h o u g h t t o have a r o l e in DNA r e p l i c a t i o n t h e i r e x a c t f unc t i on i s unknownO). Terminal DNA fragments of fowl adenovirus type 10 </ere iden t i f i ed by limited Bal 31 digest ion of genomic DNA prior t...

Multiplex polymerase chain reaction (PCR) refers to the simultaneous amplification of multiple regions of deoxyribonucleic acid (DNA) using PCR. Commercial short tandem repeat (STR) assays that can coamplify as many as 16 different loci have become widely used in forensic DNA typing. This chapter will focus on some of the aspects of constructing robust STR multiplex assays, including careful de...